دانلود کتاب Molecular Biotechnology Principles and Applications of Recombinant DNA 5th Edition
خرید ایبوک Molecular Biotechnology Principles and Applications of Recombinant DNA 5th Edition
برای دانلود کتاب Molecular Biotechnology Principles and Applications of Recombinant DNA نسخه 5 ام و خرید ایبوک مولکولی بیوتکنولوژی اصول و کاربردها از DNA نوترکیب بر روی کلید خرید در انتهای صفحه کلیک کنید. پس از اتصال به درگاه پرداخت و تکمیل مراحل خرید، لینک دانلود ایمیل می شود. کتاب 5th Edition در قالب PDF کاملا اورجینال و به زبان انگلیسی بلافاصله و بدون واترمارک یا صفحات تبلیغاتی در بین صفحات کتاب ارسال می شود. کتاب بیوتکنولوژی مولکولی : اصول و کاربرد DNA نوترکیب امکان جستجو و کپي متن و همچنين ذخيره تصاوير دارد.
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Molecular Biotechnology: Principles and Applications of Recombinant DNA (ASM Books) 5th Edition by Bernard R. Glick (Author), Cheryl L. Patten (Author)
دانلود رایگان ایبوک Molecular Biotechnology Principles and Applications of Recombinant DNA
برای اطمینان از کیفیت کتاب Molecular Biotechnology Principles and Applications ، چند صفحه ابتدایی ان بصورت رایگان قرار داده شده است. کتاب بیوتکنولوژی مولکولی : اصول و کاربرد DNA نوترکیب
درباره کتاب Molecular Biotechnology Principles and Applications of Recombinant DNA 5th Edition
Since 1994, Molecular Biotechnology: Principles and Applications of Recombinant DNA has introduced students to the fast-changing world of molecular biotechnology. With each revision, the authors have extensively updated the book to keep pace with the many new techniques in gene isolation and amplification, nucleic acid synthesis and sequencing, gene editing, and their applications to biotechnology. In this edition, authors Bernard R. Glick and Cheryl L. Patten have continued that tradition, but have also overhauled the book’s organization to
Detail fundamental molecular biology methods and recombinant protein engineering techniques, which provides students with a solid scientific basis for the rest of the book. Present the processes of molecular biotechnology and its successes in medicine, bioremediation, raw material production, biofuels, and agriculture. Examine the intersection of molecular biotechnology and society, including regulation, patents, and controversies around genetically modified products. Filled with engaging figures that strongly support the explanations in the text, Molecular Biotechnology: Principles and Applications of Recombinant DNA presents difficult scientific concepts and technically challenging methods in clear, crisp prose.
This excellent textbook is ideal for undergraduate and graduate courses in introductory biotechnology, as well as, courses dedicated to medical, agricultural, environmental, and industrial biotechnology applications.
دانلود کتاب مولکولی بیوتکنولوژی : اصول و کاربردها از DNA نوترکیب نسخه پنجم
از سال 1994 ، بیوتکنولوژی مولکولی: اصول و کاربردهای DNA نوترکیب دانشجویان را به دنیای سریع در حال تغییر بیوتکنولوژی مولکولی معرفی کرده است. با هر نسخه ، نویسندگان به طور گسترده ای کتاب را به روز کرده اند تا با بسیاری از تکنیک های جدید در جداسازی و تقویت ژن ، سنتز و توالی اسید نوکلئیک ، ویرایش ژن و کاربردهای آنها در بیوتکنولوژی همگام باشد. در این نسخه ، نویسندگان برنارد آر. گلیك و چریل ال پاتن این سنت را ادامه داده اند ، اما سازمان كتاب را نیز مورد بررسی قرار داده اند.
جزئیات روش های اساسی زیست شناسی مولکولی و تکنیک های مهندسی پروتئین نوترکیب را شرح دهید ، که پایه علمی محکمی را برای بقیه کتاب در اختیار دانشجویان قرار می دهد. فرآیندهای بیوتکنولوژی بیولوژیکی مولکولی و موفقیتهای آن در پزشکی ، تجمع زیستی ، تولید مواد اولیه ، سوخت های زیستی و کشاورزی را ارائه دهید. تقاطع بیوتکنولوژی مولکولی و جامعه ، از جمله مقررات ، اختراعات ثبت اختراعات و اختلاف نظرها درباره محصولات اصلاح شده ژنتیکی را بررسی کنید.
پر از چهره های جذاب که به شدت از توضیحات موجود در متن پشتیبانی می کنند ، بیوتکنولوژی مولکولی: اصول و کاربردهای DNA نوترکیب مفاهیم علمی دشوار و روشهای فنی چالش برانگیز را در نثر واضح و واضح ارائه می دهد.
این کتاب درسی عالی برای دوره های کارشناسی و کارشناسی ارشد در بیوتکنولوژی مقدماتی و همچنین دوره های اختصاصی به برنامه های کاربردی بیوتکنولوژی پزشکی ، کشاورزی ، زیست محیطی و صنعتی مناسب است.
فهرست مطالب PDF کتاب Molecular Biotechnology Principles and Applications نسخه 5
Table Of Contents Cover Contents Preface to the Fifth Edition 1. The Development of Molecular Biotechnology Emergence of Molecular Biotechnology Recombinant DNA Technology Commercialization of Molecular Biotechnology Concerns and Consequences Summary References Review Questions 2. Fundamental Technologies Molecular Cloning Preparation of DNA for Cloning Insertion of Target DNA into a Plasmid Vector Transformation and Selection of Cloned DNA in a Bacterial Host Cloning Eukaryotic Genes Recombinational Cloning Genomic Libraries Genome Engineering Using CRISPR Technology Polymerase Chain Reaction Amplification of DNA Using PCR Cloning PCR Products Quantitative PCR Chemical Synthesis of DNA Synthesis of Oligonucleotides Assembling Oligonucleotides into Genes Gene Synthesis by PCR DNA Sequencing Technologies Dideoxynucleotide Procedure Pyrosequencing Sequencing Using Reversible Chain Terminators Sequencing by Single Molecule Synthesis Sequencing Whole Genomes Preparation of Genomic DNA Sequencing Libraries High-Throughput Next-Generation Sequencing Strategies Genome Sequence Assembly Sequencing Metagenomes Genomics Transcriptomics DNA Microarrays RNA Sequencing Proteomics Identification of Proteins Protein Expression Profiling Protein−Protein Interactions Metabolomics Summary References Review Questions 3. Production of Recombinant Proteins Protein Production in Prokaryotic Hosts Regulation of Transcription Increasing Translation Efficiency Increasing Protein Stability Facilitating Protein Folding Decreasing Protein Degradation Increasing Protein Secretion Secretion into the Periplasm Secretion into the Medium Facilitating Protein Purification DNA Integration into the Host Chromosome Heterologous Protein Production in Eukaryotic Cells Posttranslational Modification of Eukaryotic Proteins General Features of Eukaryotic Expression Systems Yeast Expression Systems Saccharomyces cerevisiae Expression Vectors Secretion of Heterologous Proteins by S. cerevisiae Other Yeast Expression Systems Baculovirus–Insect Cell Expression Systems Baculovirus Expression Vectors Mammalian Glycosylation and Processing of Proteins in Insect Cells Mammalian Cell Expression Systems Vector Design Selectable Markers for Mammalian Expression Vectors Engineering Mammalian Cell Hosts for Enhanced Productivity Chromosomal Integration and Environment Protein Engineering Directed Mutagenesis Site-Directed Mutagenesis by Overlap Extension PCR Site-Directed Mutagenesis by Inverse PCR Mutant Proteins with Unusual Amino Acids Random Mutagenesis Error-Prone PCR Random Insertion/Deletion Mutagenesis Random Mutagenesis with Degenerate Oligonucleotide Primers DNA Shuffling Examples of Protein Engineering Increasing Protein Stability Modifying Protein Specificity Modifying Cofactor Requirements Decreasing Protease Sensitivity Summary References Review Questions 4. Molecular Diagnostics Immunological Approaches to Detect Protein Biomarkers Antibodies Structure of Antibodies Polyclonal Antibodies Monoclonal Antibodies Agglutination Enzyme-Linked Immunosorbent Assays Measuring Disease-Associated Proteins by Sandwich ELISA Diagnosing Autoimmune Diseases using an Indirect ELISA Protein Arrays to Detect Polygenic Diseases Immunoassays for Protein Conformation-Specific Disorders DNA-Based Diagnostic Approaches Hybridization Probes Allele-Specific Hybridization Oligonucleotide Ligation Assay Padlock Probes Molecular Beacons PCR-Based Detection Methods Allele-Specific PCR TaqMan PCR for SNP Genotypiing Real-Time PCR to Detect Infectious Disease Forensic Analysis Using PCR-Amplified Microsatellites DNA Microarrays Detection of Multiple Alleles Detection of Epigenetic Markers Ancestry Determination Animal Species Determination Whole Genome Sequencing to Assess Genetic Disease Risk Detecting RNA Signatures of Disease Detection of Disease-Associated Changes in Gene Expression Using Microarrays Detection of RNA Signatures of Antibiotic Resistance in Bacteria Detection of miRNA Signatures of Cancers Biofluorescent and Bioluminescent Systems Fluorescent Proteins Luciferase Microbial Biosensors Summary References Review Questions 5. Protein Therapeutics Pharmaceuticals Human Interferons Human Growth Hormone Tumor Necrosis Factor Alpha Extending Protein Half-Life Enzymes DNase I Alginate Lyase Phenylalanine Ammonia Lyase α1-Antitrypsin Glycosidases Masking Nonhuman Epitopes Engineered Bacteriophages Targeting Mitochondria Lactic Acid Bacteria Interleukin-10 Leptin An HIV Inhibitor Insulin Recombinant Antibodies Hybrid Human–Mouse Monoclonal Antibodies Human Monoclonal Antibodies Antibody Fragments Combinatorial Libraries of Antibody Fragments A Combinatorial Library of Full-Length Antibodies Shuffling CDR Sequences Dual-Variable Domain Antibodies Anticancer Antibodies Antibodies Against Various Diseases Antiobesity Antibodies Enhanced Antibody Half-Life Summary References Review Questions 6. Nucleic Acids as Therapeutic Agents Targeting Specific mRNA and DNA Sequences Antisense RNA Aptamers Ribozymes and DNAzymes Interfering RNA Zinc Finger Nucleases CRISPR-Cas System Nanozymes Nanoparticles Viral Delivery Systems Nonviral Delivery Systems Direct Injection Lipids Bacteria Dendrimers Antibodies Aptamers Transposons Gene Therapy Prodrug Activation Therapy Promoterless Gene Targeting Summary References Review Questions 7. Vaccines Vaccination Current and Future Vaccines Subunit Vaccines Herpes Simplex Virus Cholera SARS Staphylococcus aureus Human Papillomavirus Foot-and-Mouth Disease Streptococcus Delivery Peptide Vaccines Malaria Genetic Immunization: DNA Vaccines Delivery Cancer Dental Caries Attenuated Vaccines Herpes Simplex Virus Cholera Salmonella Species Leishmania Species Vector Vaccines Vaccines Directed against Viruses Dengue Virus Ebola Virus Vaccines Directed against Bacteria Tuberculosis Bacteria as Antigen Delivery Systems Cholera Helicobacter pylori Monoclonal Antibody Passive Immunity Influenza Virus Summary References Review Questions 8. Industrial and Environmental Uses of Recombinant Microorganisms Restriction Endonucleases Small Biological Molecules L-Ascorbic Acid Indigo Amino Acids L-Cysteine L-Valine L-Citrulline Modified Corynebacterium Lycopene Antibiotics Antibiotic Biosynthesis Genes Engineering Polyketide Antibiotics Unique Antibiotics Overproducing Antibiotics Biopolymers Xanthan Gum Polyhydroxyalkanoates Microbial Degradation of Xenobiotics Genetic Engineering of Biodegradative Pathways Manipulation by Transfer of Plasmids Manipulation by Gene Alteration Utilization of Starch and Sugars Commercial Production of Fructose and Alcohol Increasing Alcohol Production Alcohol Tolerance Improving Fructose Production Utilization of Cellulose and Hemicellulose Lignocellulosics Cellulase Genes Direct Conversion of Biomass to Ethanol Zymomonas mobilis Lipids from Cyanobacteria Hydrogen Production Summary References Review Questions 9. Large-Scale Production of Proteins from Recombinant Microorganisms Principles of Microbial Growth Batch Fermentation Fed-Batch Fermentation Continuous Fermentation Maximizing the Efficiency of the Fermentation Process High-Density Cell Cultures Increasing Plasmid Stability Quiescent E. Coli Cells Protein Secretion Reducing Acetate Bioreactors Typical Large-Scale Fermentation Systems Two-Stage Fermentation in Tandem Airlift Reactors Two-Stage Fermentation in a Single Stirred-Tank Reactor Batch versus Fed-Batch Fermentation Harvesting Microbial Cells Disrupting Microbial Cells Downstream Processing Protein Solubilization Utilizing an Immobilized Enzyme Magnetic Separation of Proteins Large-Scale Production of Plasmid DNA Summary References Review Questions 10. Genetic Engineering of Plants: Methodology Plant Transformation with the Ti Plasmid of A. Tumefaciens Ti Plasmid–Derived Vector Systems Microprojectile Bombardment Chloroplast Engineering Very High Level Protein Expression Use of Reporter Genes in Transformed Plant Cells Manipulation of Gene Expression in Plants Transient Gene Expression Plant Promoters Targeted Gene Editing Facilitating Protein Purification Oleosins Rhizosecretion Protein Glycosylation Production of Marker-Free Transgenic Plants Removing Marker Genes from Nuclear DNA Removing Marker Genes from Chloroplast DNA Summary References Review Questions 11. Transgenic Plants Insect Resistance B. thuringiensis Insecticidal Toxin Increasing Expression of the B. thuringiensis Protoxin Other Strategies for Protecting Plants against Insects Protease Inhibitors α-Amylase Inhibitor Cholesterol Oxidase Vegetative Insecticidal Toxins Other Proteins RNA Interference Preventing the Development of B. thuringiensis–Resistant Insects Targeting Aphids Virus Resistance Viral Coat Protein–Mediated Protection Protection by Expression of Other Genes Single-Chain Antibodies Micro-RNAs CRISPR-Cas Herbicide Resistance Fungus and Bacterium Resistance Salt and Drought Stress Fruit Ripening and Flower Wilting Modification of Plant Nutritional Content Amino Acids Lipids Vitamins Vitamin A Folate Iron Gluten Modification of Food Plant Taste and Appearance Preventing Discoloration Starch Plants as Bioreactors Antibodies Poly(3-hydroxybutyric Acid) Edible Vaccines Plant Yield Altering Lignin Content Increasing Oxygen Content Summary References Review Questions 12. Transgenic Animals Transgenic Mice: Methodology DNA Microinjection Method Retroviral Vector Method Engineered Embryonic Stem Cell Method Conditional Gene Inactivation with the Cre-loxP Recombination System Genome Editing with the CRISPR-Cas System Gene Knockdown by RNA Interference Transgenic Mice: Applications Transgenic Disease Models: Alzheimer Disease Transgenic Mice as Test Systems Control of Transgene Expression Conditional Control of Cell Death Transgenic Livestock Cloning Livestock by Somatic Cell Nuclear Transfer Production of Pharmaceuticals Production of Donor Organs Disease Resistant Livestock Improving Milk Quality Improving Animal Production Traits Transgenic Poultry Transgenic Fish Summary References Review Questions 13. Molecular Biotechnology and Society Development of Guidelines for Recombinant DNA Research Deliberate Release of Genetically Modified Microorganisms Environmental Concerns Regulations Regulation of Genetically Modified Foods Food Ingredients Produced by Genetically Engineered Microorganisms Genetically Modified Crops Genetically Engineered Livestock Societal Concerns About Genetically Modified Foods Alteration of Nutritional Content of Food Potential for Introducing Toxins or Allergens into Food Potential for Transferring Transgenes from Food to Humans or Intestinal Microorganisms Controversy About the Labeling of Genetically Modified Foods Impact of Genetically Engineered Crops on Biodiversity Who Benefits from the Production of Genetically Modified Foods? Environmental Benefits of Genetically Modified Crops How do Views about Genetically Modified Organisms Impact Trade? Regulation and Safety of Medical Products of Biotechnology New Biological Drugs Genetic and Genomic Testing Economic Issues Patenting Biotechnology Patenting Patenting in Different Countries Patenting Nucleic Acid Sequences Patenting Living Organisms Patenting and Fundamental Research Summary References Review Questions Amino Acids of Proteins and Their Designations Index