دسترسی به ASBC Methods of Analysis

American Society of Brewing Chemists چیست؟

The ASBC is a professional organization of scientists and technical professionals in the brewing, malting, and allied industries. It publishes a journal, the Journal of the American Society of Brewing Chemists, usually four times a year.

انجمن امریکایی Brewing شیمی

The American Society of Brewing Chemists disseminates scientific brewing knowledge, improves and expands methods of measurement, identifies an

d rapidly responds to industry technical concerns, and develops scientists for the brewing industry.

دسترسی به ASBC Methods of Analysis

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Table of Contents

Barley Methods

Glossary [Released 2007]
VIEW GLOSSARY

Barley 1. Sampling and Grading [Released 1958, Revised 2003]
VIEW SUMMARY | VIEW METHOD

Barley 2. Physical Tests  
VIEW SUMMARY | VIEW METHOD

A. Variety [Released 1977, Revised 2003]
B. Test Weight per Bushel (Bushelweight) [Released 1958, Revised 2003]
C. Assortment [Released 1958, Revised 2003]
D. 1,000-Kernel Weight  [Released 1958, Revised 2003]
E. Texture of Endosperm (Mellowness) [Released 1958, Revised 2003]
F. Skinned and/or Broken Kernels [Released 1958, Revised 2003]
G. Weathering and Kernel Damage [Released 1958, Revised 2003]
H. Injury by Sprout [Released 1958, Revised 2003]

Barley 3. Germination
VIEW SUMMARY | VIEW METHOD

A. Germinative Energy [Released 1958]
B. Germinative Capacity — Hydrogen Peroxide Method (International Method) [Released 1978]
C. Germinative Energy, Germinative Capacity, and Water Sensitivity — Simultaneous Determination [Released 1997]

Barley 4. Preparation of Sample for Chemical Analysis [Released 1958]
VIEW SUMMARY | VIEW METHOD

Barley 5. Moisture
VIEW SUMMARY | VIEW METHOD

A. Air-Oven Method  [Released 1958]
B. Alternate Air-Oven Method  [Released 1979]
C. Moisture in Whole Grain by Near-Infrared  [Released 1998]

Barley 6. Extract  [Released 1958]
VIEW SUMMARY | VIEW METHOD

Barley 7. Protein [Release date 2007]
VIEW SUMMARY | VIEW METHOD

A. Protein (N x 6.25) by Kjeldahl (International Method) [Released 1958]
B. Protein by Near-Infrared [Release date 1980]
C. Protein (N x 6.25) by Combustion [Released 1992]
D. Protein in Whole Grain by Near-Infrared [Released 1998]

Barley 8. Potential Diastatic Power [Released 1959]
VIEW SUMMARY | VIEW METHOD

Barley 9. Kernel Brightness (Instrumental) [Released 1979]
VIEW SUMMARY | VIEW METHOD

Barley 10. Barley Pregermination by Fluorescein Dibutyrate (International Method) [Released 1989]
VIEW SUMMARY | VIEW METHOD

Barley 11. Deoxynivalenol by Gas Chromatography [Released 1996]
VIEW SUMMARY | VIEW METHOD

Barley 12. Sprout Damage
VIEW SUMMARY | VIEW METHOD

A. By Falling Number  [Released 2004]
B. By Stirring Number [Released 2004]

Malt Methods

Glossary [Released 2011]
VIEW GLOSSARY

Malt 1. Sampling  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Malt 2. Physical Tests  
VIEW SUMMARY | VIEW METHOD

A. Test Weight per Bushel (Bushelweight) [Released 2011]
B. Assortment  [Released 2011]
C. 1,000-Kernel Weight  [Released 2011]
D. Foreign Seeds and Skinned or Broken Kernels  [Released 2011]
E. Mealiness  [Release date 2011]
F. Length of Acrospire (Growth Count)  [Released 2011]

Malt 3. Moisture  [Released 1958]
VIEW SUMMARY | VIEW METHOD

Malt 4. Extract  [Released 1958, revised 2011]
VIEW SUMMARY | VIEW METHOD

Malt 6. Diastatic Power  
VIEW SUMMARY | VIEW METHOD

A. Diastatic Power  [Released 2011]
B. Diastatic Power (Rapid Method)  [Released 2011]
C. Automated Flow Analysis  [Released 2011]

Malt 7. Alpha-Amylase  
VIEW SUMMARY | VIEW METHOD

A. Fixed Color and Variable Time Method (International Method)  [Released 2011]
B. Fixed Time and Variable Color Method  [Released 2011]
C. Automated Flow Analysis Method Using Iodine Reagent [Released 2011]
D. Automated Flow Analysis Method Using Potassium Ferricyanide Reagent  [Released 2013]

Malt 8. Protein (N X 6.25)  
VIEW SUMMARY | VIEW METHOD

A. By Kjeldahl (International Method)  [Released 2011]
B. By Combustion  [Release 2011]

Malt 9. High-Dried, Caramel, and Black Malts (Caramel Extract is International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Malt 10. N-Nitrosamines  
VIEW SUMMARY | VIEW METHOD

A. Vacuum Distillation Method  [Released 2011]
B. Celite Column Extraction  [Released 2011]
C. Hot Aqueous Extraction  [Released 2011]

Malt 11. Sulfur Dioxide  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Malt 12. Malt Modification by Friability (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Malt 13. Deoxynivalenol by Gas Chromatography  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Malt 14. Dimethyl Sulfide Precursor by Headspace Gas Chromatography
[Released 2011]
VIEW SUMMARY | VIEW METHOD

Malt 15. Grist  
VIEW SUMMARY | VIEW METHOD

A. By Standard Sieve  [Released 2011]
B. By Manual Sieve  [Released 2011]

Malt 16. Lipoxygenase Enzymatic Activity In Malted Barley By Absorbance Method  [Released 2017]
VIEW SUMMARY | VIEW METHOD

Adjunct Materials Methods

Glossary [Released 2007]
VIEW GLOSSARY

Introduction
VIEW INTRODUCTION

Cereals

Cereals 1. Sampling  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Cereals 2. Physical Characteristics  
VIEW SUMMARY | VIEW METHOD

Removal of Accidental Foreign Particles  [Release date 1958]
Color  [Release date 1958]
Odor  [Release date 1958]
Husks, Germs, and Foreign Seeds  [Release date 1958]
Mold  [Release date 1958]
Insect Infestation  [Release date 1958]
Assortment of Corn Grits  [Release date 1963]

Cereals 3. Moisture (International Method)  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Cereals 4. Oil (Fatty Substances)  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Cereals 5. Extract  
VIEW SUMMARY | VIEW METHOD

A. Malt Conversion Method (International Method)  [Release date 1958]
B. Enzyme Conversion Method for Corn Grits (International Method)  [Release date 1963]

Cereals 6. Protein (N X 6.25)  
VIEW SUMMARY | VIEW METHOD

A. By Kjeldahl  [Release date 1958]
B. By Combustion  [Release date 1992]

Cereals 7. Ash  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups

Sugars and Syrups 1. Sampling  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups 2. Physical Characteristics (Color, Odor, Taste)  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups 3. Clarity of “10% Solution”*  [Release date 1958]

Sugars and Syrups 4. Color of “10% Solution” (International Method)*  [Release date 1958]

Sugars and Syrups 5. Extract (International Method)  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups 6. Moisture  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups 7. Fermentable Extract  
VIEW SUMMARY | VIEW METHOD

A. By Regular Fermentation  [Release date 1958]
B. By Rapid Fermentation 1958 [Release date 1958]

Sugars and Syrups 8. Iodine Reaction  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups 9. Acidity  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups 10. pH (Hydrogen Ion Concentration)  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups 11. Protein (N X 6.25)  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups 12. Ash  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups 13. Diastatic Power (Diastatic Malt Syrups Only)  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups 14. Total Reducing Sugars  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups 15. Sucrose  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups 16. Dextrose in Presence of Other Reducing Sugars  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Sugars and Syrups 17. Fermentable Saccharides by Chromatography  
VIEW SUMMARY | VIEW METHOD

A. Gas Chromatography Method  [Release date 1977]
B. High-Performance Liquid Chromatography Method 1977 [Release date 1977]

Sugars and Syrups 18. Fermentable Carbohydrates by Cation Exchange HPLC  [Release date 1997]
VIEW SUMMARY | VIEW METHOD

Brewers’ Grains Methods

Glossary [Release date 2007]
VIEW GLOSSARY

Brewers’ Grains 1. Sampling  
VIEW SUMMARY | VIEW METHOD

A. Wet Brewers’ Grains  [Release date 1958]
B. Dry Brewers’ Grains  [Release date 1958

Brewers’ Grains 2. Preparation of Sample  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Brewers’ Grains 3. Moisture  
VIEW SUMMARY | VIEW METHOD

A. On Sample in Wet Condition  [Release date 1958]
B. On Sample After Preliminary Drying  [Release date 1958]
C. On Dry Brewer’ Grains  [Release date 1958]
D. On Sample in Wet Condition—Rapid Method  [Release date 1995

Brewers’ Grains 4. Available Extract  
VIEW SUMMARY | VIEW METHOD

A. Wet Brewers’ Grains  [Release date 1958]
B. Dry Brewers’ Grains  [Release date 1958]

Brewers’ Grains 5. Soluble Extract  
VIEW SUMMARY | VIEW METHOD

A. Wet Brewers’ Grains  [Release date 1958]
B. Dry Brewers’ Grains  [Release date 1958]

Brewers’ Grains 6. Feed Analysis  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Brewers’ Grains 7. Protein (N x 6.25) by Combustion  [Release date 1992]
VIEW SUMMARY | VIEW METHOD

Hops Methods

Glossary [Release date 2007]
VIEW GLOSSARY

Hops 1. Sampling  [Release date 2010]
VIEW SUMMARY | VIEW METHOD

Hops 2. Physical Examination  [Release date 2010]
VIEW SUMMARY | VIEW METHOD

Hops 3. Aphids in Hops  [Release date 2010]
VIEW SUMMARY | VIEW METHOD

Hops 4. Moisture  
VIEW SUMMARY | VIEW METHOD

A. Moisture by Distillation  [Release date 2010]
B. Moisture by Vacuum Drying  [Release date 2010]
C. Moisture by Routine Air Oven Method  [Release date 2010]

Hops 5. Resins  [Release date 2010]
VIEW SUMMARY | VIEW METHOD

Hops 6. α-and β-Acids in Hops and Hop Pellets  
VIEW SUMMARY | VIEW METHOD

A. α-and β-Acids by Spectrophotometry  [Release date 2010]
B. Conductometric Value  [Release date 2010]

Hops 7. α-Acids in Hops and Hop Pellets by Ion-Exchange Chromatography*  

A. α-Acids Using Sephadex Ion-Exchange Resin*  [Release date 1976]
B. α-Acids Using Dowex Ion-Exchange Resin*  [Release date 1976]

Hops 8. Non-Isomerized Hop Extracts  
VIEW SUMMARY | VIEW METHOD

A. Isooctane Spectrophotometric Method*  [Release date 1970]
B. Isopropyl Ether Spectrophotometric and Conductometric Methods  [Release date 2010]

Hops 9. Isomerized Hop Extracts  
VIEW SUMMARY | VIEW METHOD

A. Iso-α-Acids and rho-Iso-α-Acids by Dowex Ion-Exchange Chromatography* 1974  [Release date 1958]
B. Iso-α-Acids by Sephadex Stepwise Ion-Exchange Chromatography (International Method)* 1979  [Release date 1958]
C. Iso-α-Acids by HPLC  [Release date 2010]
D. Iso-α-Acids by HPLC  [Release date 2010]

Hops 10. Hop Bitter Acids in Non-Isomerized Extracts by Stepwise Ion-Exchange Chromatography*  

A. α-Acids by Dowex Ion-Exchange Chromatography*  [Release date 1977]
B. α-Acids by Sephadex Ion-Exchange Chromatography*  [Release date 1977]
C. β-Acids by Sephadex Ion-Exchange Chromatography*  [Release date 1978]

Hops 11. Gradient Elution of Hop Constituents Using Sephadex Ion-Exchange Chromatography*  [Release date 1978]

Hops 12. Hop Storage Index  [Release date 2010]
VIEW SUMMARY | VIEW METHOD

Hops 13. Total Essential Oils in Hops and Hop Pellets by Steam Distillation  [Release date 2010]
VIEW SUMMARY | VIEW METHOD

Hops 14. α-Acids and β-Acids in Hops and Hop Extracts by HPLC (International Method)  [Release date 2010]
VIEW SUMMARY | VIEW METHOD

Hops 15. Iso-α-Acids in Isomerized Hop Pellets by HPLC 1993  [Release date 2010]
VIEW SUMMARY | VIEW METHOD

Hops 16. Iso-α, α-, and β-Acids in Hop Extracts and Isomerized Hop Extracts by HPLC  [Release date 2010]
VIEW SUMMARY | VIEW METHOD

Hops 17. Hop Essential Oils by Capillary Gas Chromatography-Flame Ionization Detection  [Release date 2004]
VIEW SUMMARY | VIEW METHOD

Hops 18. Determintion Of Tetrahydroiso-α-Acids In Hop Products By Spectrophotometry [Release date 2017]
VIEW SUMMARY | VIEW METHOD

Wort Methods

Glossary [Release date 2011]
VIEW GLOSSARY

Wort 1. Sampling  [Release date 2011]
VIEW SUMMARY | VIEW METHOD

Wort 2. Specific Gravity  
VIEW SUMMARY | VIEW METHOD

A. By Pycnometer*  [Release date 1958]
B. By Digital Density Meter  [Release date 2011]

Wort 3. Extract  [Release date 2011]
VIEW SUMMARY | VIEW METHOD

Wort 4. Apparent Extract by Hydrometer  [Release date 2011]
VIEW SUMMARY | VIEW METHOD

Wort 5. Yeast Fermentable Extract  [Release date 2011]
VIEW SUMMARY | VIEW METHOD

Wort 6. Iodine Reaction*  [Release date 1958]

Wort 7. Total Acidity  [Release date 2011]
VIEW SUMMARY | VIEW METHOD

Wort 8. pH (Hydrogen Ion Concentration)  [Release date 2011]
VIEW SUMMARY | VIEW METHOD

Wort 9. Wort Color and Sample Preparation  
VIEW SUMMARY | VIEW METHOD

A. Celite  [Release date 2011]
B. Filter Paper or Disks  [Release date 2011]

Wort 10. Protein  
VIEW SUMMARY | VIEW METHOD

A. By Kjeldahl  [Release date 2011]
B. By Combustion  [Release date 2011]

Wort 11. Reducing Sugars (Copper Reducing Substances)  [Release date 2011]
VIEW SUMMARY | VIEW METHOD

Wort 12. Free Amino Nitrogen (International Method)  [Release date 2011]
VIEW SUMMARY | VIEW METHOD

A. Ninhydrin Method  [Release date 2011]
B. Segmented Flow Analysis [Release date 2011]

Wort 13. Viscosity
VIEW SUMMARY | VIEW METHOD

A. Viscometer Method  [Release date 2010]
B. Rolling Ball Method  [Release date 2013]

Wort 14. Fermentable Saccharides by Chromatography  
VIEW SUMMARY | VIEW METHOD

A. Gas Chromatography Method  [Release date 2011]
B. High-Performance Liquid Chromatography Method (International Method)  [Release date 2011]

Wort 15. Magnesium by Atomic Absorption Spectrophotometry  [Release date 2011]
VIEW SUMMARY | VIEW METHOD

Wort 16. Zinc by Atomic Absorption Spectrophotometry (International Method)  [Release date 2011]
VIEW SUMMARY | VIEW METHOD

Wort 17. Protein in Unhopped Wort by Spectrophotometry  [Release date 2011]
VIEW SUMMARY | VIEW METHOD

Wort 18. β-Glucan in Congress Wort [Release date 2011]
VIEW SUMMARY | VIEW METHOD

A. Fluorescence [Release date 2011]
B. Segmented Flow Analysis [Release date 2011]

Wort 19. Fermentable Carbohydrates by Cation Exchange HPLC  [Release date 2011]
VIEW SUMMARY | VIEW METHOD

Wort 20. Elemental Analysis by Inductively Coupled Plasma-Atomic Emission Spectroscopy  [Release date 2011]
VIEW SUMMARY | VIEW METHOD

Wort 21. Thiobarbituric Acid Index 2009  [Release date 2010]
VIEW SUMMARY | VIEW METHOD

Wort 22. Wort and Beer Fermentable and Total Carbohydrates by HPLC [Release date 2012]
VIEW SUMMARY | VIEW METHOD

Wort 23. Wort Bitterness [Release date 2012]
VIEW SUMMARY | VIEW METHOD

A. Bitterness Units by Spectrophotometry [Release date 2011]
B. Bitterness Units by Segmented Flow Analysis [Release date 2011]
C. Iso-α-Acids in Wort by HPLC [Release date 2011]

Wort 24. International Bitterness Units in Wort by Spectrophotometer
VIEW SUMMARY

Wort 25. Rapid Malt Color [Release date 2016]
VIEW SUMMARY | VIEW METHOD

Beer Methods

Recommended Beer Degassing Methods and Alternatives Matrix
VIEW SUMMARY | VIEW MATRIX

Recommended Beer Degassing Methods and Alternatives – Comparison Chart
VIEW SUMMARY | VIEW CHART

Glossary [Release date 2006]
VIEW GLOSSARY

Beer 1. Sampling  
VIEW SUMMARY | VIEW METHOD

A. Preparation of Sample for Chemical and Physical Analysis  [Release date 1958]
B. Aseptic Sampling from Tanks and Lines  [Release date 1966]
C. Aseptic Sampling from Finished Packages  [Release date 1971]
D. Decarbonation by a Rotary Shaker  [Release date 1998]

Beer 2. Specific Gravity
Reviewed 2014
VIEW SUMMARY | VIEW METHOD

A. By Pycnometer*  [Released 1958; Revised 1975; Archived 1992]
B. By Digital Density Meter  [Released 1978; Revised 1981, reviewed and revised 2014]

Beer 3. Apparent Extract  [Release date 1958, revised 1975, reviewed and revised 2014]
VIEW SUMMARY | VIEW METHOD

Beer 4. Alcohol  
VIEW SUMMARY | VIEW METHOD

A. Beer and Distillate Measured Volumetrically  [Release date 1958]
B. Beer and Distillate Measured Gravimetrically  [Release date 1958]
C. Alcohol Determined Refractometrically  [Release date 1965]
D. Ethanol Determined by Gas Chromatography  [Release date 1979]
E. Instrumental Method for Alcohol and Original Gravity Content  [Release date 1989]
F. Enzymatic Method for Low Alcohol Concentrations (International Method)  [Release date 1991]
G. Near-Infrared and Original Extract Content  [Release date 2004]

Beer 5. Real Extract  
VIEW SUMMARY | VIEW METHOD

A. Beer Measured Volumetrically  [Release date 1958, Reviewed 2018]
B. Beer Measured Gravimetrically  [Release date 1958, Reviewed 2018]
C. Real Extract Determined Refractometrically  [Release date 1982, Reviewed 2018]

Beer 6. Calculated Values  
VIEW SUMMARY | VIEW METHOD

A. Extract of Original Wort  [Release date 1958, Reviewed 2018]
B. Real Degree of Fermentation  [Release date 1958, Reviewed 2018]
C. Apparent Degree of Fermentation  [Release date 1958, Reviewed 2018]
D. Carbohydrate Content of Beer  [Release date 1978, Reviewed 2018]

Beer 7. Iodine Reaction*

A. For Pale Beers*  [Release date 1958]
B. For Dark Beer but Also Applicable to Light Beers*  [Release date 1958]

Beer 8. Total Acidity  
VIEW SUMMARY | VIEW METHOD

A. By Potentiometric Titration  [Release date 1958, reviewed 2016]
B. By Titration of Diluted Beer with Phenolphthalein as Indicator  [Release date 1958, reviewed 2016]

Beer 9. pH (Hydrogen Ion Concentration)  [Release date 1958]
VIEW SUMMARY | VIEW METHOD

Beer 10. Color  
VIEW SUMMARY | VIEW METHOD

A. Spectrophotometric Color Method  [Release date 1958, revised 1975, reviewed 2015]
B. Photometric Method (Instruments other than precision spectrophotometers)  [Release date 1958, revised 1975, reviewed 2015]
C. Tristimulus Analysis (Colorimetric or Spectrophotometric)  [Release date 2002, reviewed 2015]

Beer 11. Protein   
VIEW SUMMARY | VIEW METHOD

A. By Kjeldahl  [Release date 1958, revised 1975, 1990, reviewed 2015]
B. By Combustion  [Release date 1993, reviewed 2015]
C. By Spectrophotometer  [Release date 2005, revised 2013, reviewed 2015]

Beer 12. Reducing Sugars (Copper Reducing Substances)  
VIEW SUMMARY | VIEW METHOD

A. Munson and Walker Method  [Release date 1958]
B. Lane-Eynon Volumetric Method  [Release date 1958]

Beer 13. Dissolved Carbon Dioxide  
VIEW SUMMARY | VIEW METHOD

A. Pressure Method for Beer in Tanks  [Release date 1958]
B. Pressure Method for Beer in Bottles and Cans  [Release date 1958]
C. Manometric/Volumetric Method  [Release date 1993]
D. Volume Expansion Method  [Release date 2009]

Beer 14. Ash 1958 [Release date 1958, revised 1975, reviewed 2015]
VIEW SUMMARY | VIEW METHOD

Beer 15. Total Phosphorus*  [Release date 1958]

Beer 16. End Fermentation (Yeast Fermentable Extract)  [Released 1958, revised 1975, reviewed 2015]
VIEW SUMMARY | VIEW METHOD

Beer 17. Dextrins*  [Released 1958]

Beer 18. Iron 
VIEW SUMMARY | VIEW METHOD

A. Analysis by Colorimetry (International)  [Released 1958; Revised 1975, reviewed 2015]
B. Analysis by Atomic Absorption Spectrophotometry (International)  [Released 1974; Revised 1975, reviewed 2015]
C. Analysis by Ferrozine  [Released 1992; reviewed and revised 2015]

Beer 19. Copper*  

A. By ZDBT Method (International Method)  [Release date 1958]*
B. Cuprethol Method 1969 [Release date 1978]*
C. Analysis by Atomic Absorption Spectrophotometry (International Method)  [Release date 1974]*

Beer 20. Calcium 
VIEW SUMMARY | VIEW METHOD

A. Chrome Black T Indicator Method  [Release date 1972, revised 1975, reviewed and revised 2015]
B. Calcein Indicator Method (Direct Titration)  [Release date 1972, reviewed and revised 2015]
C. Analysis by Atomic Absorption Spectrophotometry (International Method)  [Released 1972, revised 1975, reviewed and revised 2015]

Beer 21. Total Sulfur Dioxide
VIEW SUMMARY | VIEW METHOD

A. p-Rosaniline Method  [Release date 1975]
B. Segmented Flow Analyzer Method (International Method) [Release date 2009]

Beer 22. Foam Collapse Rate 
VIEW SUMMARY | VIEW METHOD

A. Sigma Value Method (Modified Carlsberg Method)  [Release date 1962]
B. Foam Flashing Method  [Release date 1962]

Beer 23. Beer Bitterness 
VIEW SUMMARY | VIEW METHOD

A. Bitterness Units (International Method)  [Release date 1968, revised 1975] B. ISO-α-Acids by Solvent Extraction  [Release date 1968, revised 2011]
C. ISO-α-Acids by Solid-Phase Extraction and HPLC  [Release date 1993]
D. Bitterness Units by Automated Flow Analysis  [Release date 1995]
E. ISO-α-Acids in Beer by HPLC  [Release date 2011]
F. ISO-α-Acids (IAA) [Release date 2011]

Beer 24. n-Heptyl p-Hydroxybenzoate* 

A. By Gas-Liquid Chromatography*  [Release date 1972]
B. By Ultraviolet Spectrophotometry (Nonspecific Method)*  [Release date 1972]

Beer 25. Diacetyl 
VIEW SUMMARY | VIEW METHOD

A. Macro Dimethyl Glyoxime Method*  [Release date 1964]
B. Broad Spectrum Method for VDK  [Release date 1964]
C. Micro Dimethyl Glyoxime Method  [Release date 1964]
D. Ultraviolet Spectrophotometer Method*  [Release date 1964]
E. Gas Chromatographic Method*  [Release date 1964]
F. Gas Chromatographic Method B

Beer 26. Formazin Turbidity Standards  [Release date 1958, revised 1975, reviewed and revised 2015]
VIEW SUMMARY | VIEW METHOD

Beer 27. Physical Stability 
VIEW SUMMARY | VIEW METHOD

I. Total Haze after Chilling
A. Visual Method  [Release date 1962]
B. Nephelometric Method  [Release date 1962]
II. Accelerated Chill-Haze Test
A. Elevated Temperature Storage Prior to Chilling  [Release date 1965]

Beer 28. Qualitative Test for Detection of Chillproofing Enzymes in Beer*  [Release date 1967]

Beer 29. Lower Boiling Volatiles in Beer or Ale  [Release date 1973]
VIEW SUMMARY | VIEW METHOD

Beer 30. Testing for Taste Difference Between Two Beers  [Release date 1969]
VIEW SUMMARY | VIEW METHOD

Beer 31. Free Amino Nitrogen (International Method)  [Release date 1975]
VIEW SUMMARY | VIEW METHOD

Beer 32. Viscosity (International Method)  [Release date 1975]
VIEW SUMMARY | VIEW METHOD

Beer 33. Caloric Content (Calculated)  [Release date 1970]
VIEW SUMMARY | VIEW METHOD

Beer 34. Dissolved Oxygen  [Release date 1976]
VIEW SUMMARY | VIEW METHOD

Beer 35. Total Polyphenols (International Method)  [Released 1978, reviewed and revised 2015]
VIEW SUMMARY | VIEW METHOD

Beer 36. Sodium by Atomic Absorption Spectrophotometry (International Method)
[Release date 1978, revised 1981, reviewed and revised 2015]
VIEW SUMMARY | VIEW METHOD

Beer 37. Potassium by Atomic Absorption Spectrophotometry (International Method)  [Released 1978, revised 1981, reviewed and revised 2015]
VIEW SUMMARY | VIEW METHOD

Beer 38. Magnesium and Calcium 
VIEW SUMMARY | VIEW METHOD

A. Magnesium by Atomic Absorption Spectrophotometry (International Method)  [Release date 1982]
B. Calcium and Magnesium by Sequential Titration  [Release date 1985]

Beer 39. Chloride 
VIEW SUMMARY | VIEW METHOD

A. Potentiometer Titration*  [Released 1980, archived 1992]
B. Conductometric Titration (International Method)  [Released 1980, reviewed 2015]
C. Mercurimetric Titration  [Released 1980, reviewed and revised 2015]

Beer 40. N-Nitrosamines 
VIEW SUMMARY | VIEW METHOD

A. Distillation  [Release date 1981, Reviewed 2018]
B. Celite Adsorption  [Release date 1965, Reviewed 2018]

Beer 41. Total Carbohydrate 
VIEW SUMMARY | VIEW METHOD

A. By Spectrophotometry  [Released 1983]
B. By HPLC  [Released 1990]

Beer 42. Aluminum by Graphite Furnace Atomic Absorption Spectrophotometry  [Released 1992, reviewed and revised 2016]
VIEW SUMMARY | VIEW METHOD

Beer 43. Anions (Chloride, Phosphate, and Sulfate) by Ion Chromatography (International Method)  [Released 1994, Reviewed 2018]
VIEW SUMMARY | VIEW METHOD

Beer 44. Dimethyl Sulfide by Chemiluminescence Detection
[Released 1994, reviewed and revised 2015]
VIEW SUMMARY | VIEW METHOD

Beer 45. Elemental Analysis by Inductively Coupled Plasma-Atomic Emission Spectroscopy  [Released 2007]
VIEW SUMMARY | VIEW METHOD

Beer 46. Measurement of Oxidative Resistance in Beer by Electron Paramagnetic Resonance  [Released 2009, reviewed and revised 2016]
VIEW SUMMARY | VIEW METHOD

Beer 47. Iso-Alpha-Acids In Beer and Wort by HPLC
VIEW SUMMARY

Beer 48. Headspace Gas Chromatography–Flame Ionization Detection Analysis of Beer Volatiles [Released 2013]
VIEW SUMMARY | VIEW METHOD

Beer 49. Determination of Gluten Using the R5 Competitive ELISA Method [Released 2013]
VIEW SUMMARY | VIEW METHOD

Beer 50. Foam Stability by Nibem [Released 2016]
VIEW SUMMARY | VIEW METHOD

Beer Inclusions: Common Causes of Elevated Turbidity  [Released 2008]
VIEW SUMMARY | VIEW METHOD

Flavored Alcohol Beverages

Glossary  [Release date 2007]
VIEW GLOSSARY

Flavored Alcohol Beverages 1. Alcohol and Original Extract Content in Flavored Alcohol Beverages by Near-Infrared [Released 2007]
VIEW SUMMARY | VIEW METHOD

Microbiology Methods

Glossary  [Released 2007]
VIEW GLOSSARY

Introduction  [Released 2007]
VIEW INTRODUCTION

Yeast

Yeast 1. Sampling  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Yeast 2. Yeast Examination 
VIEW SUMMARY | VIEW METHOD

A. Cell Morphology  [Released 2011]
B. Presence of Bacteria  [Released 2011]

Yeast 3. Yeast Stains 
VIEW SUMMARY | VIEW METHOD

A. Dead Yeast Cell Stain (International Method)  [Released 2011]
B. Yeast Spore Stain  [Released 2011]
C. Triphenyltetrazolium Chloride for Identification of Respiratory-Deficient Cells  [Released 2011]

Yeast 4. Microscopic Yeast Cell Counting  [Released 2011, reviewed 2016]
VIEW SUMMARY | VIEW METHOD

Yeast 5. Yeast Solids 
VIEW SUMMARY | VIEW METHOD

A. Total by Dry Weight  [Released 2011]
B. Percent by Spin-Down  [Released 2011]

Yeast 6. Yeast Viability by Slide Culture  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Yeast 7. Yeast Sporulation  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Yeast 8. Killer Yeast Identification  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Yeast 9. Morphology of Giant Yeast Colonies (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Yeast 10. Differentiation of Ale and Lager Yeast 
VIEW SUMMARY | VIEW METHOD

A. X-α-GAL Medium  [Released 2011]
B. Growth at 37°C  [Released 2011]
C. Growth on Melibiose  [Released 2011]

Yeast 11. Flocculation 
VIEW SUMMARY | VIEW METHOD

A. Helm Assay  [Released 2011]
B. Absorbance Method  [Released 2011]

Yeast 12. Vitality By Fluorescence  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Yeast 13. Differentiation of Brewing Yeast Strains by PCR Fingerprinting  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Yeast 14. Miniature Fermentation Assay [Released 2012]
VIEW SUMMARY | VIEW METHOD

Yeast 15. Differentiation of Ale and Lager Yeast Strains by Rapid X-α-GAL Analysis [Released 2013]
VIEW SUMMARY | VIEW METHOD

Yeast 16. Phenolic Yeast Detection By Decarboxylation Of Ferulic Acid [Released 2016]
VIEW SUMMARY | VIEW METHOD

Microbiological Control

Microbiological Control 1. Aseptic Sampling 
VIEW SUMMARY | VIEW METHOD

A. Beer in Process  [Released 2011]
B. Packaged Beer  [Released 2011]
C. Process Water  [Released 2011]
D. Brewery Air  [Released 2011]
E. Process Equipment and Surfaces  [Released 2011]
F. Swab Surface Hygiene Using ATP Bioluminescence  [Released 2011]

Microbiological Control 2. Detection of Microorganisms 
VIEW SUMMARY | VIEW METHOD

A. Incubation  [Released 2011]
B. Plating  [Released 2011]
C. Membrane Filtration  [Released 2011]

Microbiological Control 3. Differential Staining  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Microbiological Control 4. General Culture Media 
VIEW SUMMARY | VIEW METHOD

A. Universal Beer Agar Medium  [Released 2011]
B. Brewers Tomato Juice Agar Medium  [Released 2011]

Microbiological Control 5. Differential Culture Media 
VIEW SUMMARY | VIEW METHOD

A. Cycloheximide Medium  [Released 2011]
B. Lee’s Multi-Differential Agar  [Released 2011]
C. Raka-Ray Lactic Acid Bacteria Medium  [Released 2011]
D. Lysine Medium  [Release date 2011]
E. Lin’s Wild Yeast Differential Medium  [Released 2011]
F. Barney-Miller Brewer Medium  [Released 2011]
G. DeMan Rogosa Sharpe Medium  [Released 2011]
H. MYGP + Copper Medium  [Released 2011]
I. CLEN Medium for Wild Yeast  [Released 2011]
J. Selective Medium for Megasphaera and Pectinatus (SMMP)  [Released 2011]
K. Nystatin in Selective Media  [Released 2011]
L. Hsu’s Lactobacillus and Pediococcus Medium [Released 2009]

Microbiological Control 6. Water and Rinse Water Hygiene Using ATP [Released 2012]
VIEW SUMMARY | VIEW METHOD

Common Brewery-Related Microorganisms  [Released 2008]
VIEW SUMMARY | VIEW METHOD

Filter Aids Methods

Filter Aids 1. Sampling  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Filter Aids 2. pH of Water Suspension (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Filter Aids 3. Effect on Odor and Taste (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Filter Aids 4. Iron Pickup by Beer (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Packages and Packaging Materials Methods

Introduction
VIEW INTRODUCTION

Bottles

Bottles 1. Dimensions 1999 
VIEW SUMMARY | VIEW METHOD

A. Height  [Released 1999]
B. Outside Diameter  [Released 1999]
C. Out-of-Perpendicular  [Released 1999]
D. Identification Marks  [Released 1999]
E. Glass Distribution  [Released 1999]
F. Weight 1999 [Release date 1963]
G. Locking Ring “A” Diameter  [Released 1999]
H. Reinforcing Ring “B” Diameter  [Released 1999]
I.  Width of Locking Ring  [Released 1999]
J. Throat “I” Diameter  [Released 1999]
K. Finish  [Released 1999]

Bottles 2. Defects  [Released 1999]
VIEW SUMMARY | VIEW METHOD

Bottles 3. Color 
VIEW SUMMARY | VIEW METHOD

A. Amber Color  [Released 1999]
B. Redness Ratio  [Released 1999]

Bottles 4. Capacity 
VIEW SUMMARY | VIEW METHOD

A. Overflow  [Released 1999]
B. Fill Point  [Released 1999]

Bottles 5. Surface Protective Coatings 
VIEW SUMMARY | VIEW METHOD

A. Lubricity  [Released 1999]
B. Coating Quality  [Released 1999]
C. Rub Tests  [Released 1999]

Bottle Closures

Bottle Closures 1. Defects Glossary and Classification  [Released 1999]
VIEW SUMMARY | VIEW METHOD

Bottle Closures 2. Test Pressure 
VIEW SUMMARY | VIEW METHOD

A. Internal Pressure Test for Crowns  [Released 1995]
B. Internal Pressure Test for 28-mm Topside Pilferproof Closures  [Released 1995]
C. Internal Pressure Test for Plastic-Lined Convenience Two-Way Crown Applied to GPI 500 Series Finishes  [Released 1995]
D. Secure Seal Tester (SST) Internal Pressure Test  [Released 1995]

Bottle Closures 3. Gas Retention Capability of Crowns  [Released 1968]
VIEW SUMMARY | VIEW METHOD

Bottle Closures 4. Lithography Resistance to Pasteurization Conditions for Steel Crowns and Roll-On Pilferproof Aluminum Closures [Released 1999]
VIEW SUMMARY | VIEW METHOD

Bottle Closures 5. Removal Torque Procedure 
VIEW SUMMARY | VIEW METHOD

A. For Aluminum Closures  [Releasd 1993]
B. For Crowns  [Released 1993]

Bottle Closures 6. Crimp Determination Test — Crowns  [Released 1994]
VIEW SUMMARY | VIEW METHOD

Cans

Cans 1. Defects Classification and Glossary for Seamless Two-Piece Cans 
VIEW SUMMARY | VIEW METHOD

A. Can and End Defects Classification  [Released 2004]
B. Can Defects  [Released 1999]
C. End Defects  [Released 1999]

Cans 2. Evaluation of Rusting Tendency of Beer Cans*  [Released 1970]

Cans 3. Dimensions 
VIEW SUMMARY | VIEW METHOD

A. Metal Gauge Thickness  [Released 2004]
B. Flange Width  [Released 2004]
C. Filled Can Countersink Depth  [Released 2004]

Cans 4. Ends 
VIEW SUMMARY | VIEW METHOD

A. Curl Opening  [Released 2004]
B. Seaming Chuck Fit  [Released 2004]
C. Ring-Pull-End Pop and Pull Test  [Released 2004]

Cans 5. Capacity 
VIEW SUMMARY | VIEW METHOD

A. Overflow  [Released 2004]
B. Headspace  [Released 2004]

Cans 6. Enamel Rater for Evaluating Metal Exposure  [Released 2004]
VIEW SUMMARY | VIEW METHOD

Cans 7. Beverage Can Terminology  [Released 2009]
VIEW SUMMARY | VIEW METHOD

Cans 8. Copper Sulfate Test  [Released 2009]
VIEW SUMMARY | VIEW METHOD

Fills

Fills 1. Total Contents of Bottles and Cans by Calculation from Measured Net Weight  [Released 1991]
VIEW SUMMARY | VIEW METHOD

Fills 2. Total Contents of Cans of Known Tare Weight  [Released 1991]
VIEW SUMMARY | VIEW METHOD

By-Products Methods

Introduction
VIEW INTRODUCTION

Barley

By-Products, Barley 1. Sampling  [Released 1999]
VIEW SUMMARY | VIEW METHOD

By-Products, Barley 2. Physical Tests  [Released 1999]
VIEW SUMMARY | VIEW METHOD

By-Products, Barley 3. Moisture  [Released 1999]
VIEW SUMMARY | VIEW METHOD

By-Products, Barley 4. Protein  [Released 1999]
VIEW SUMMARY | VIEW METHOD

Malt

By-Products, Malt 1. Sampling  [Released 1999]
VIEW SUMMARY | VIEW METHOD

By-Products, Malt 2. Physical Tests  [Released 1999]
VIEW SUMMARY | VIEW METHOD

By-Products, Malt 3. Moisture  [Released 1999]
VIEW SUMMARY | VIEW METHOD

By-Products, Malt 4. Protein  [Released 1999]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis Methods

Introduction
VIEW INTRODUCTION

Beer Flavor Database  [Released 2011]
VIEW SUMMARY  |   VIEW SPREADSHEET (Excel)  |   VIEW SPREADSHEET (PDF)

Sensory Analysis 1. Terms and Definitions (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis 2. Test Room, Equipment, Conduct of Test (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis 3. Choice of Method (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis 4. Selection and Training of Assessors (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis 5. Author Guidelines for Reporting (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis 6. Paired Comparison Test (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis 7. Triangular Test (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis 8. Duo-Trio Test (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis 9. Threshold of Added Substances — Ascending Method of Limits Test (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis 10. Descriptive Analysis (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis 11. Ranking Test (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis 12. Flavor Terminology and Reference Standards (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis 13. Difference-from-Control (International Method)  [Released 2011]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis 14. Hot Steep Malt Sensory Evaluation Method  [Released 2017]
VIEW SUMMARY | VIEW METHOD

Sensory Analysis 15. Hop Tea Sensory Method  [Released 2017]
VIEW SUMMARY | VIEW METHOD

Statistical Analysis Methods

Introduction
VIEW INTRODUCTION

Statistical Analysis 1. Linear Calibration  [Released 1983]
VIEW SUMMARY | VIEW METHOD

Statistical Analysis 2. Limits of Detection and Determination  [Released 1983]
VIEW SUMMARY | VIEW METHOD

Statistical Analysis 3. Ruggedness Testing  [Released 1983]
VIEW SUMMARY | VIEW METHOD

Statistical Analysis 4. Youden Unit Block Collaborative Testing Procedure  [Released 1983]
VIEW SUMMARY | VIEW METHOD

Statistical Analysis 5. Comparison of Test Methods  [Released 1986]
VIEW SUMMARY | VIEW METHOD

Statistical Analysis 6. Comparison of Microbiological Data  [Released 1987]
VIEW SUMMARY | VIEW METHOD

Appendix I – t-Distribution (Two-tailed Tests)
VIEW SUMMARY | VIEW APPENDIX

Appendix II – F-Distribution, = 0.05
VIEW SUMMARY | VIEW APPENDIX

Appendix III – F-Distribution, = 0.025
VIEW SUMMARY | VIEW APPENDIX

Appendix IV – F-Distribution, = 0.01
VIEW SUMMARY | VIEW APPENDIX

Appendix V – F-Distribution, = 0.005
VIEW SUMMARY | VIEW APPENDIX

Appendix VI – F-Distribution, = 0.001
VIEW SUMMARY | VIEW APPENDIX

Appendix VII – Cumulative Distribution of Chi-Square
VIEW SUMMARY | VIEW APPENDIX

Appendix VIII – Definition of Statistical Terms  [Released 1987]
VIEW SUMMARY | VIEW APPENDIX

Appendix Procedure

I Instrument Standardization 
VIEW APPENDIX

A. Calibration of Ultraviolet-Visible Spectrophotometer (International Method)  [Released 1972]

II Nomenclature of Hop Resin Fractions and Components  [Released 1969]
VIEW APPENDIX

III Water Analysis  [Released 1971]
VIEW APPENDIX

Tables

Tables for Extract Determinations in Malt and Cereals
VIEW SUMMARY | VIEW TABLES

Tables Related to Determinations on Wort, Beer, and Brewing Sugars and Syrups
VIEW SUMMARY | VIEW TABLES

Table 1. Extract

Table 2. Alcohol

Table 3. Original Extract (correction factors)

Table 4. Reducing Substances (Munsun-Walker)

Table 5. Reducing Substances (Lane-Eynon)

Table 6. Baume Degrees

Identification Guides

Beer Inclusions: Common Causes of Elevated Turbidity
VIEW SUMMARY | VIEW GUIDE

Common Brewery-Related Microorganisms
VIEW SUMMARY | VIEW GUIDE

Flavor Standard Spiking Calculator
VIEW SUMMARY | VIEW CALCULATOR

Beer Flavor Database  [Released 2011]
VIEW SUMMARY  |   VIEW SPREADSHEET (Excel)  |   VIEW SPREADSHEET (PDF)

Hop Flavor Database
VIEW SUMMARY  |   VIEW SPREADSHEET (Excel)   |   VIEW SPREADSHEET (PDF)

Recommended Reading
VIEW SUMMARY | VIEW DOCUMENT