دانلود کتاب Molecular Biotechnology Principles and Applications of Recombinant DNA 5th Edition

خرید ایبوک Molecular Biotechnology Principles and Applications of Recombinant DNA 5th Edition

برای دانلود کتاب Molecular Biotechnology Principles and Applications of Recombinant DNA نسخه 5 ام و خرید ایبوک مولکولی بیوتکنولوژی اصول و کاربردها از DNA نوترکیب بر روی کلید خرید در انتهای صفحه کلیک کنید. پس از اتصال به درگاه پرداخت و تکمیل مراحل خرید، لینک دانلود ایمیل می شود.  کتاب 5th Edition در قالب PDF کاملا اورجینال بلافاصله و بدون واترمارک یا صفحات تبلیغاتی در بین صفحات کتاب ارسال می شود. ایبوک امکان جستجو و کپي متن و همچنين ذخيره تصاوير دارد.

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خرید ایبوک Molecular Biotechnology Principles and Applications of Recombinant DNA 5th Edition دانلود کتاب اصول بیوتکنولوژی مولکولی و برنامه های کاربردی DNA 5 نوترکیب نسخه 5
خرید ایبوک Molecular Biotechnology Principles and Applications of Recombinant DNA 5th Edition دانلود کتاب اصول بیوتکنولوژی مولکولی و برنامه های کاربردی DNA 5 نوترکیب نسخه 5

Molecular Biotechnology: Principles and Applications of Recombinant DNA (ASM Books) 5th Edition
by Bernard R. Glick (Author), Cheryl L. Patten (Author)

Series: ASM Books

Hardcover: 740 pages
Publisher: ASM Press; 5 edition (June 20, 2017)
Language: English
ISBN-10: 1555819362
ISBN-13: 9781555819361

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دانلود رایگان ایبوک Molecular Biotechnology Principles and Applications of Recombinant DNA

برای اطمینان از کیفیت کتاب Molecular Biotechnology Principles and Applications ، چند صفحه ابتدایی ان بصورت رایگان قرار داده شده است.

 درباره کتاب Molecular Biotechnology Principles and Applications of Recombinant DNA 5th Edition

Since 1994, Molecular Biotechnology: Principles and Applications of Recombinant DNA has introduced students to the fast-changing world of molecular biotechnology. With each revision, the authors have extensively updated the book to keep pace with the many new techniques in gene isolation and amplification, nucleic acid synthesis and sequencing, gene editing, and their applications to biotechnology. In this edition, authors Bernard R. Glick and Cheryl L. Patten have continued that tradition, but have also overhauled the book’s organization to

Detail fundamental molecular biology methods and recombinant protein engineering techniques, which provides students with a solid scientific basis for the rest of the book.
Present the processes of molecular biotechnology and its successes in medicine, bioremediation, raw material production, biofuels, and agriculture.
Examine the intersection of molecular biotechnology and society, including regulation, patents, and controversies around genetically modified products.
Filled with engaging figures that strongly support the explanations in the text, Molecular Biotechnology: Principles and Applications of Recombinant DNA presents difficult scientific concepts and technically challenging methods in clear, crisp prose.

This excellent textbook is ideal for undergraduate and graduate courses in introductory biotechnology, as well as, courses dedicated to medical, agricultural, environmental, and industrial biotechnology applications.

دانلود کتاب مولکولی بیوتکنولوژی : اصول و کاربردها از DNA نوترکیب نسخه پنجم

از سال 1994 ، بیوتکنولوژی مولکولی: اصول و کاربردهای DNA نوترکیب دانشجویان را به دنیای سریع در حال تغییر بیوتکنولوژی مولکولی معرفی کرده است. با هر نسخه ، نویسندگان به طور گسترده ای کتاب را به روز کرده اند تا با بسیاری از تکنیک های جدید در جداسازی و تقویت ژن ، سنتز و توالی اسید نوکلئیک ، ویرایش ژن و کاربردهای آنها در بیوتکنولوژی همگام باشد. در این نسخه ، نویسندگان برنارد آر. گلیك و چریل ال پاتن این سنت را ادامه داده اند ، اما سازمان كتاب را نیز مورد بررسی قرار داده اند.

جزئیات روش های اساسی زیست شناسی مولکولی و تکنیک های مهندسی پروتئین نوترکیب را شرح دهید ، که پایه علمی محکمی را برای بقیه کتاب در اختیار دانشجویان قرار می دهد.
فرآیندهای بیوتکنولوژی بیولوژیکی مولکولی و موفقیتهای آن در پزشکی ، تجمع زیستی ، تولید مواد اولیه ، سوخت های زیستی و کشاورزی را ارائه دهید.
تقاطع بیوتکنولوژی مولکولی و جامعه ، از جمله مقررات ، اختراعات ثبت اختراعات و اختلاف نظرها درباره محصولات اصلاح شده ژنتیکی را بررسی کنید.

پر از چهره های جذاب که به شدت از توضیحات موجود در متن پشتیبانی می کنند ، بیوتکنولوژی مولکولی: اصول و کاربردهای DNA نوترکیب مفاهیم علمی دشوار و روشهای فنی چالش برانگیز را در نثر واضح و واضح ارائه می دهد.

این کتاب درسی عالی برای دوره های کارشناسی و کارشناسی ارشد در بیوتکنولوژی مقدماتی و همچنین دوره های اختصاصی به برنامه های کاربردی بیوتکنولوژی پزشکی ، کشاورزی ، زیست محیطی و صنعتی مناسب است.

فهرست مطالب PDF کتاب Molecular Biotechnology Principles and Applications نسخه 5

Table Of Contents
Cover
Contents
Preface to the Fifth Edition
1. The Development of Molecular Biotechnology
Emergence of Molecular Biotechnology
Recombinant DNA Technology
Commercialization of Molecular Biotechnology
Concerns and Consequences
Summary
References
Review Questions
2. Fundamental Technologies
Molecular Cloning
Preparation of DNA for Cloning
Insertion of Target DNA into a Plasmid Vector
Transformation and Selection of Cloned DNA in a Bacterial Host
Cloning Eukaryotic Genes
Recombinational Cloning
Genomic Libraries
Genome Engineering Using CRISPR Technology
Polymerase Chain Reaction
Amplification of DNA Using PCR
Cloning PCR Products
Quantitative PCR
Chemical Synthesis of DNA
Synthesis of Oligonucleotides
Assembling Oligonucleotides into Genes
Gene Synthesis by PCR
DNA Sequencing Technologies
Dideoxynucleotide Procedure
Pyrosequencing
Sequencing Using Reversible Chain Terminators
Sequencing by Single Molecule Synthesis
Sequencing Whole Genomes
Preparation of Genomic DNA Sequencing Libraries
High-Throughput Next-Generation Sequencing Strategies
Genome Sequence Assembly
Sequencing Metagenomes
Genomics
Transcriptomics
DNA Microarrays
RNA Sequencing
Proteomics
Identification of Proteins
Protein Expression Profiling
Protein−Protein Interactions
Metabolomics
Summary
References
Review Questions
3. Production of Recombinant Proteins
Protein Production in Prokaryotic Hosts
Regulation of Transcription
Increasing Translation Efficiency
Increasing Protein Stability
Facilitating Protein Folding
Decreasing Protein Degradation
Increasing Protein Secretion
Secretion into the Periplasm
Secretion into the Medium
Facilitating Protein Purification
DNA Integration into the Host Chromosome
Heterologous Protein Production in Eukaryotic Cells
Posttranslational Modification of Eukaryotic Proteins
General Features of Eukaryotic Expression Systems
Yeast Expression Systems
Saccharomyces cerevisiae Expression Vectors
Secretion of Heterologous Proteins by S. cerevisiae
Other Yeast Expression Systems
Baculovirus–Insect Cell Expression Systems
Baculovirus Expression Vectors
Mammalian Glycosylation and Processing of Proteins in Insect Cells
Mammalian Cell Expression Systems
Vector Design
Selectable Markers for Mammalian Expression Vectors
Engineering Mammalian Cell Hosts for Enhanced Productivity
Chromosomal Integration and Environment
Protein Engineering
Directed Mutagenesis
Site-Directed Mutagenesis by Overlap Extension PCR
Site-Directed Mutagenesis by Inverse PCR
Mutant Proteins with Unusual Amino Acids
Random Mutagenesis
Error-Prone PCR
Random Insertion/Deletion Mutagenesis
Random Mutagenesis with Degenerate Oligonucleotide Primers
DNA Shuffling
Examples of Protein Engineering
Increasing Protein Stability
Modifying Protein Specificity
Modifying Cofactor Requirements
Decreasing Protease Sensitivity
Summary
References
Review Questions
4. Molecular Diagnostics
Immunological Approaches to Detect Protein Biomarkers
Antibodies
Structure of Antibodies
Polyclonal Antibodies
Monoclonal Antibodies
Agglutination
Enzyme-Linked Immunosorbent Assays
Measuring Disease-Associated Proteins by Sandwich ELISA
Diagnosing Autoimmune Diseases using an Indirect ELISA
Protein Arrays to Detect Polygenic Diseases
Immunoassays for Protein Conformation-Specific Disorders
DNA-Based Diagnostic Approaches
Hybridization Probes
Allele-Specific Hybridization
Oligonucleotide Ligation Assay
Padlock Probes
Molecular Beacons
PCR-Based Detection Methods
Allele-Specific PCR
TaqMan PCR for SNP Genotypiing
Real-Time PCR to Detect Infectious Disease
Forensic Analysis Using PCR-Amplified Microsatellites
DNA Microarrays
Detection of Multiple Alleles
Detection of Epigenetic Markers
Ancestry Determination
Animal Species Determination
Whole Genome Sequencing to Assess Genetic Disease Risk
Detecting RNA Signatures of Disease
Detection of Disease-Associated Changes in Gene Expression Using Microarrays
Detection of RNA Signatures of Antibiotic Resistance in Bacteria
Detection of miRNA Signatures of Cancers
Biofluorescent and Bioluminescent Systems
Fluorescent Proteins
Luciferase
Microbial Biosensors
Summary
References
Review Questions
5. Protein Therapeutics
Pharmaceuticals
Human Interferons
Human Growth Hormone
Tumor Necrosis Factor Alpha
Extending Protein Half-Life
Enzymes
DNase I
Alginate Lyase
Phenylalanine Ammonia Lyase
α1-Antitrypsin
Glycosidases
Masking Nonhuman Epitopes
Engineered Bacteriophages
Targeting Mitochondria
Lactic Acid Bacteria
Interleukin-10
Leptin
An HIV Inhibitor
Insulin
Recombinant Antibodies
Hybrid Human–Mouse Monoclonal Antibodies
Human Monoclonal Antibodies
Antibody Fragments
Combinatorial Libraries of Antibody Fragments
A Combinatorial Library of Full-Length Antibodies
Shuffling CDR Sequences
Dual-Variable Domain Antibodies
Anticancer Antibodies
Antibodies Against Various Diseases
Antiobesity Antibodies
Enhanced Antibody Half-Life
Summary
References
Review Questions
6. Nucleic Acids as Therapeutic Agents
Targeting Specific mRNA and DNA Sequences
Antisense RNA
Aptamers
Ribozymes and DNAzymes
Interfering RNA
Zinc Finger Nucleases
CRISPR-Cas System
Nanozymes
Nanoparticles
Viral Delivery Systems
Nonviral Delivery Systems
Direct Injection
Lipids
Bacteria
Dendrimers
Antibodies
Aptamers
Transposons
Gene Therapy
Prodrug Activation Therapy
Promoterless Gene Targeting
Summary
References
Review Questions
7. Vaccines
Vaccination
Current and Future Vaccines
Subunit Vaccines
Herpes Simplex Virus
Cholera
SARS
Staphylococcus aureus
Human Papillomavirus
Foot-and-Mouth Disease
Streptococcus
Delivery
Peptide Vaccines
Malaria
Genetic Immunization: DNA Vaccines
Delivery
Cancer
Dental Caries
Attenuated Vaccines
Herpes Simplex Virus
Cholera
Salmonella Species
Leishmania Species
Vector Vaccines
Vaccines Directed against Viruses
Dengue Virus
Ebola Virus
Vaccines Directed against Bacteria
Tuberculosis
Bacteria as Antigen Delivery Systems
Cholera
Helicobacter pylori
Monoclonal Antibody Passive Immunity
Influenza Virus
Summary
References
Review Questions
8. Industrial and Environmental Uses of Recombinant Microorganisms
Restriction Endonucleases
Small Biological Molecules
L-Ascorbic Acid
Indigo
Amino Acids
L-Cysteine
L-Valine
L-Citrulline
Modified Corynebacterium
Lycopene
Antibiotics
Antibiotic Biosynthesis Genes
Engineering Polyketide Antibiotics
Unique Antibiotics
Overproducing Antibiotics
Biopolymers
Xanthan Gum
Polyhydroxyalkanoates
Microbial Degradation of Xenobiotics
Genetic Engineering of Biodegradative Pathways
Manipulation by Transfer of Plasmids
Manipulation by Gene Alteration
Utilization of Starch and Sugars
Commercial Production of Fructose and Alcohol
Increasing Alcohol Production
Alcohol Tolerance
Improving Fructose Production
Utilization of Cellulose and Hemicellulose
Lignocellulosics
Cellulase Genes
Direct Conversion of Biomass to Ethanol
Zymomonas mobilis
Lipids from Cyanobacteria
Hydrogen Production
Summary
References
Review Questions
9. Large-Scale Production of Proteins from Recombinant Microorganisms
Principles of Microbial Growth
Batch Fermentation
Fed-Batch Fermentation
Continuous Fermentation
Maximizing the Efficiency of the Fermentation Process
High-Density Cell Cultures
Increasing Plasmid Stability
Quiescent E. Coli Cells
Protein Secretion
Reducing Acetate
Bioreactors
Typical Large-Scale Fermentation Systems
Two-Stage Fermentation in Tandem Airlift Reactors
Two-Stage Fermentation in a Single Stirred-Tank Reactor
Batch versus Fed-Batch Fermentation
Harvesting Microbial Cells
Disrupting Microbial Cells
Downstream Processing
Protein Solubilization
Utilizing an Immobilized Enzyme
Magnetic Separation of Proteins
Large-Scale Production of Plasmid DNA
Summary
References
Review Questions
10. Genetic Engineering of Plants: Methodology
Plant Transformation with the Ti Plasmid of A. Tumefaciens
Ti Plasmid–Derived Vector Systems
Microprojectile Bombardment
Chloroplast Engineering
Very High Level Protein Expression
Use of Reporter Genes in Transformed Plant Cells
Manipulation of Gene Expression in Plants
Transient Gene Expression
Plant Promoters
Targeted Gene Editing
Facilitating Protein Purification
Oleosins
Rhizosecretion
Protein Glycosylation
Production of Marker-Free Transgenic Plants
Removing Marker Genes from Nuclear DNA
Removing Marker Genes from Chloroplast DNA
Summary
References
Review Questions
11. Transgenic Plants
Insect Resistance
B. thuringiensis Insecticidal Toxin
Increasing Expression of the B. thuringiensis Protoxin
Other Strategies for Protecting Plants against Insects
Protease Inhibitors
α-Amylase Inhibitor
Cholesterol Oxidase
Vegetative Insecticidal Toxins
Other Proteins
RNA Interference
Preventing the Development of B. thuringiensis–Resistant Insects
Targeting Aphids
Virus Resistance
Viral Coat Protein–Mediated Protection
Protection by Expression of Other Genes
Single-Chain Antibodies
Micro-RNAs
CRISPR-Cas
Herbicide Resistance
Fungus and Bacterium Resistance
Salt and Drought Stress
Fruit Ripening and Flower Wilting
Modification of Plant Nutritional Content
Amino Acids
Lipids
Vitamins
Vitamin A
Folate
Iron
Gluten
Modification of Food Plant Taste and Appearance
Preventing Discoloration
Starch
Plants as Bioreactors
Antibodies
Poly(3-hydroxybutyric Acid)
Edible Vaccines
Plant Yield
Altering Lignin Content
Increasing Oxygen Content
Summary
References
Review Questions
12. Transgenic Animals
Transgenic Mice: Methodology
DNA Microinjection Method
Retroviral Vector Method
Engineered Embryonic Stem Cell Method
Conditional Gene Inactivation with the Cre-loxP Recombination System
Genome Editing with the CRISPR-Cas System
Gene Knockdown by RNA Interference
Transgenic Mice: Applications
Transgenic Disease Models: Alzheimer Disease
Transgenic Mice as Test Systems
Control of Transgene Expression
Conditional Control of Cell Death
Transgenic Livestock
Cloning Livestock by Somatic Cell Nuclear Transfer
Production of Pharmaceuticals
Production of Donor Organs
Disease Resistant Livestock
Improving Milk Quality
Improving Animal Production Traits
Transgenic Poultry
Transgenic Fish
Summary
References
Review Questions
13. Molecular Biotechnology and Society
Development of Guidelines for Recombinant DNA Research
Deliberate Release of Genetically Modified Microorganisms
Environmental Concerns
Regulations
Regulation of Genetically Modified Foods
Food Ingredients Produced by Genetically Engineered Microorganisms
Genetically Modified Crops
Genetically Engineered Livestock
Societal Concerns About Genetically Modified Foods
Alteration of Nutritional Content of Food
Potential for Introducing Toxins or Allergens into Food
Potential for Transferring Transgenes from Food to Humans or Intestinal Microorganisms
Controversy About the Labeling of Genetically Modified Foods
Impact of Genetically Engineered Crops on Biodiversity
Who Benefits from the Production of Genetically Modified Foods?
Environmental Benefits of Genetically Modified Crops
How do Views about Genetically Modified Organisms Impact Trade?
Regulation and Safety of Medical Products of Biotechnology
New Biological Drugs
Genetic and Genomic Testing
Economic Issues
Patenting Biotechnology
Patenting
Patenting in Different Countries
Patenting Nucleic Acid Sequences
Patenting Living Organisms
Patenting and Fundamental Research
Summary
References
Review Questions
Amino Acids of Proteins and Their Designations
Index
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