خرید ایبوک Precast Concrete Structures

برای دانلود کتاب Precast Concrete Structures و خرید ایبوک سازه های بتنی پیش ساخته بر روی کلید خرید در انتهای صفحه کلیک کنید. پس از اتصال به درگاه پرداخت و تکمیل مراحل خرید، لینک دانلود ایمیل می شود.  کتاب Precast Concrete Structures در قالب PDF کاملا اورجینال بلافاصله و بدون واترمارک یا صفحات تبلیغاتی در بین صفحات کتاب ارسال می شود. ایبوک امکان جستجو و کپي متن و همچنين ذخيره تصاوير دارد.

 در صورتی که نیاز به دانلود هر کتابی از آمازون یا گوگل بوک دارید، فقط کافیست ادرس اینترنتی کتاب را از سایت www.amazon.com و یا books.google.com برای ما ارسال کنید (راههای ارتباطی در صفحه تماس با گیگاپیپر ). پس از بررسی، هزینه ان اعلام می شود. پس از واریز نسخه الکترونیکی ارسال می شود.

Precast Concrete Structures (Beton-Kalender Series) 2nd Edición, Edición Kindle
de Alfred Steinle (Author), Hubert Bachmann (Author), Mathias Tillmann (Author), Philip Thrift (Translator)

ISBN-13: 978-3433032251
ISBN-10: 3433032254

لینک کتاب Precast Concrete Structures از وایلی:

https://www.wiley.com/en-us/Precast+Concrete+Structures%2C+2nd+Edition-p-9783433609040

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دانلود رایگان ایبوک Precast Concrete Structures

برای اطمینان از کیفیت کتاب Precast Concrete Structures ، چند صفحه ابتدایی ان بصورت رایگان قرار داده شده است.

 درباره کتاب Precast Concrete Structures

Building with precast concrete elements is one of the most innovative forms of construction. This book serves as an introduction to this topic, including examples, and thus supplies all the information necessary for conceptual and detailed design.

دانلود کتاب سازه های بتنی پیش ساخته

ساختمان با عناصر بتنی پیش ساخته یکی از خلاقانه ترین اشکال ساخت و ساز است. این کتاب به عنوان مقدمه ای برای این موضوع، شامل مثال ها، عمل می کند و بنابراین تمام اطلاعات لازم برای طراحی مفهومی و جزئی را فراهم می کند.

فهرست مطالب PDF کتاب Precast Concrete Structures

TABLE OF CONTENTS
Introduction 1

References 16

1 General 17

1.1 The Advantages of Factory Production 17

1.2 Historical Development 19

1.3 European Standardisation 21

References 29

2 Design of Precast Concrete Structures 31

2.1 General 31

2.2 Tolerances and Calculations for Fit 35

2.2.1 General 35

2.2.2 Tolerance Standards 36

2.2.3 Calculations for Fit 40

2.3 Production 42

2.4 Transport and Erection 46

2.4.1 General 46

2.4.2 Transport 48

2.4.3 Erection 51

2.5 Sustainability 53

2.6 Design Examples 54

References 57

3 Stability of Precast Concrete Structures 59

3.1 General 59

3.2 Loads on Stability Components 59

3.2.1 General 59

3.2.2 Wind Load Case 60

3.2.3 Out-of-Plumb Load Case 63

3.2.4 Seismic Load Case 64

3.2.5 Restraint Load Case (Shrinkage and Temperature) 69

3.3 Shear Walls for Stability 70

3.3.1 Typical Stability Elements 70

3.3.2 Arrangement of Stability Elements 74

3.4 Distribution of Horizontal Loads 80

3.4.1 General Procedure 80

3.4.2 Equations for Rough Preliminary Design 81

3.5 Analysis of Stability Components 84

3.6 Construction Details 86

3.6.1 Floor Diaphragms 86

3.6.2 Shear Walls 86

References 88

4 Precast Concrete Elements 91

4.1 General 91

4.2 Floor and Roof Elements 91

4.2.1 General 91

4.2.2 Solid Slabs 91

4.2.3 Hollow-Core Slabs 91

4.2.4 Precast Floor Plates With In Situ Concrete Topping 95

4.2.5 Ribbed Elements 98

4.2.6 Other Floor Systems 100

4.3 Beams 101

4.3.1 Purlins, Frame Beams, Downstand Beams 101

4.3.2 Roof Beams 101

4.4 Columns 103

4.5 Walls 109

4.5.1 General 109

4.5.2 Precast Concrete Wall Elements 109

4.6 Foundations 111

4.6.1 General 111

4.6.2 Monolithic Foundations 111

4.6.3 Pocket and Pad Foundations 114

4.6.4 Other Types of Foundation 118

References 119

5 Connections for Precast Concrete Construction 123

5.1 General 123

5.2 Purlin Supports 124

5.3 Roof Beam Supports 125

5.4 Floor Slab Supports 126

5.4.1 Ribbed Elements 126

5.4.2 Prestressed Hollow-Core Slabs 128

5.5 Downstand Beam Supports 128

5.6 Wall Element Supports 130

5.7 Balcony Slabs 133

5.8 Stair Supports 134

5.9 Column/Foundation 135

References 137

6 Individual Design Issues 139

6.1 General 139

6.2 Partially Loaded Areas 139

6.3 Supports 140

6.3.1 General 140

6.3.2 Elastomeric Bearings 141

6.3.3 Technical Codes of Practice for Elastomeric Bearings 143

6.3.4 Design Methods for Elastomeric Bearings 144

6.3.5 Horizontal Forces 145

6.3.6 Sizing the Bearing 146

6.3.7 Design of and Details for Supports 147

6.4 Column Butt Joints 151

6.4.1 General 151

6.4.2 Column Butt Joints with Bed of Grout (Hard Support) 152

6.4.3 Column Butt Joints with Deformable Joint Materials 155

6.4.4 Rigid Joints 156

6.4.5 Column Joints with High-Strength Steel Reinforcement 156

6.5 Wall/Floor Connections 160

6.6 Shear Dowels 161

6.6.1 General 161

6.6.2 Large Edge Distances a|| ≥8∅B or a⟂ ≥8∅B 163

6.6.3 Small Edge Distances a|| <8∅B or a⟂ <8∅B 164

6.6.4 Further Advice Concerning Shear Dowels 165

6.7 Welded Connections 166

6.8 Bolted Connections and Screw Couplers 171

6.9 Other Forms of Connection 173

6.10 Transport Anchors 173

6.10.1 General 173

6.10.2 Actions 174

6.10.3 Determining the Permissible Ultimate Resistance 176

6.10.4 Further Advice for Design 178

6.10.5 Consequences of the ‘Machinery Directive’ 179

6.10.6 Incompatibility of Transport Anchor Systems 179

6.11 Shear at the Interface Between Concrete Cast at Different Times 180

6.11.1 General 180

6.11.2 Design 180

6.11.3 Surface Categories 184

6.11.4 Construction Details 186

6.11.5 Fatigue 187

6.12 Floor Diaphragms and Shear Walls 188

6.12.1 General 188

6.12.2 Floor Diaphragms 189

6.12.3 Shear Walls 192

6.12.4 Miscellaneous 195

6.13 Shear Forces in Floor Elements 195

6.14 Half Joints 200

6.14.1 General 200

6.14.2 Design 200

6.15 Corbels 204

6.15.1 General 204

6.15.2 Design 205

6.15.3 Construction Details 213

6.15.4 Beam Nibs 214

6.15.5 Retrofitted Corbels 215

6.16 Analysis of Lateral Buckling 217

6.16.1 General 217

6.16.2 Simplified Lateral Buckling Analysis 217

6.16.3 Numerical Analysis 218

6.16.4 Analysis of Supports 226

6.17 Design for Fire 228

6.17.1 General 228

6.17.2 Principles of Design for Fire 229

6.17.3 Reinforced and Prestressed Concrete Beams 233

6.17.4 Reinforced Concrete Columns 234

6.17.5 Reinforced Non-Braced Columns 237

6.17.6 Fire Walls 237

6.17.7 Plaster and Render Finishes 238

6.17.8 Junctions, Joints, and Connections 238

6.18 Pretensioning 242

6.18.1 General 242

6.18.2 Concrete Cover 243

6.18.3 Level of Prestress 243

6.18.4 Loss of Prestress 244

6.18.5 Decompression 246

6.18.6 Stress Limitation 247

6.18.7 Transferring and Anchoring the Prestress 247

6.18.8 Tensile Splitting Forces and end Face Tension 249

References 252

7 Precast Concrete Façades 257

7.1 General 257

7.2 Conceptual Design 257

7.3 Surface Finishes 260

7.3.1 General 260

7.3.2 Surface Finishes Produced by the Moulds 261

7.3.3 Concrete Surface Treatments 261

7.3.4 Weathering Behaviour 263

7.4 Joint Waterproofing 266

7.5 Concrete Sandwich Panels 268

7.5.1 General 268

7.5.2 Dimensions and Leaf Thicknesses 268

7.5.3 Connectors 269

7.5.4 Actions 273

7.5.5 Design 278

7.5.6 Deformations 281

7.5.7 Cracking 281

7.5.8 Construction Details 282

7.6 Suspended Façade Panels 284

7.6.1 Large-Format, Suspended Façade Panels 284

7.6.2 Small-Format, Suspended Façade Panels 287

7.7 Further Developments for Concrete Façades 289

7.7.1 Textile-Reinforced Concrete 289

7.7.2 Photoconcrete 290

7.7.3 Translucent Concrete 290

7.7.4 Concrete with Glass Aggregate 290

7.7.5 Glass–Concrete Composite 292

7.8 Building Physics 292

7.8.1 Energy Considerations and Thermal Performance 292

7.8.2 Moisture Control 300

7.9 Examples 300

7.9.1 Züblin House 300

7.9.2 Community Centre in Mannheim 302

7.9.3 Ohligsmühle Office Building 302

7.9.4 Tour Total 303

7.9.5 ROC Mondriaan in The Hague 305

References 306

8 Production 311

8.1 Production Methods 311

8.1.1 General 311

8.1.2 Production Using Fixed Moulds 311

8.1.3 Pallet Circulation Systems 315

8.2 Concretes for Precast Concrete Elements 317

8.2.1 General 317

8.2.2 Fresh Concretes 320

8.2.3 Hardened Concretes 321

8.2.4 Ultra-High Performance Concretes 322

8.2.5 Self-compacting Concretes 324

8.2.6 Fibre-Reinforced Concretes 326

8.3 Heat Treatment and Curing 327

8.4 Reinforcement 329

8.4.1 General 329

8.4.2 Materials 330

8.4.3 Reinforcement Drawings 330

8.4.4 Bending and Assembling Reinforcement 332

8.5 Pretensioning in Prestressing Beds 334

8.5.1 General 334

8.5.2 Materials 335

8.5.3 Construction Documents 335

8.5.4 Production 338

8.6 Quality Assurance 342

8.6.1 General 342

8.6.2 Factory Production Control 342

8.6.3 External Monitoring 344

8.6.4 Certification and Labelling 344

References 345

Index 351

خرید ایبوک Molecular Biotechnology Principles and Applications of Recombinant DNA 5th Edition

برای دانلود کتاب Molecular Biotechnology Principles and Applications of Recombinant DNA نسخه 5 ام و خرید ایبوک مولکولی بیوتکنولوژی اصول و کاربردها از DNA نوترکیب بر روی کلید خرید در انتهای صفحه کلیک کنید. پس از اتصال به درگاه پرداخت و تکمیل مراحل خرید، لینک دانلود ایمیل می شود.  کتاب 5th Edition در قالب PDF کاملا اورجینال و به زبان انگلیسی بلافاصله و بدون واترمارک یا صفحات تبلیغاتی در بین صفحات کتاب ارسال می شود. کتاب بیوتکنولوژی مولکولی : اصول و کاربرد DNA نوترکیب امکان جستجو و کپي متن و همچنين ذخيره تصاوير دارد.

 در صورتی که نیاز به دانلود هر کتابی از آمازون یا گوگل بوک دارید، فقط کافیست ادرس اینترنتی کتاب را از سایت www.amazon.com و یا books.google.com برای ما ارسال کنید (راههای ارتباطی در صفحه تماس با گیگاپیپر ). پس از بررسی، هزینه ان اعلام می شود. پس از واریز نسخه الکترونیکی ارسال می شود.

Molecular Biotechnology: Principles and Applications of Recombinant DNA (ASM Books) 5th Edition
by Bernard R. Glick (Author), Cheryl L. Patten (Author)

Series: ASM Books

Hardcover: 740 pages
Publisher: ASM Press; 5 edition (June 20, 2017)
Language: English
ISBN-10: 1555819362
ISBN-13: 9781555819361

لینک کتاب Molecular Biotechnology Principles and Applications از وایلی:

https://www.wiley.com/en-ir/Molecular+Biotechnology:+Principles+and+Applications+of+Recombinant+DNA,+5th+Edition-p-9781555819361

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دانلود رایگان ایبوک Molecular Biotechnology Principles and Applications of Recombinant DNA

برای اطمینان از کیفیت کتاب Molecular Biotechnology Principles and Applications ، چند صفحه ابتدایی ان بصورت رایگان قرار داده شده است. کتاب بیوتکنولوژی مولکولی : اصول و کاربرد DNA نوترکیب

 درباره کتاب Molecular Biotechnology Principles and Applications of Recombinant DNA 5th Edition

Since 1994, Molecular Biotechnology: Principles and Applications of Recombinant DNA has introduced students to the fast-changing world of molecular biotechnology. With each revision, the authors have extensively updated the book to keep pace with the many new techniques in gene isolation and amplification, nucleic acid synthesis and sequencing, gene editing, and their applications to biotechnology. In this edition, authors Bernard R. Glick and Cheryl L. Patten have continued that tradition, but have also overhauled the book’s organization to

Detail fundamental molecular biology methods and recombinant protein engineering techniques, which provides students with a solid scientific basis for the rest of the book.
Present the processes of molecular biotechnology and its successes in medicine, bioremediation, raw material production, biofuels, and agriculture.
Examine the intersection of molecular biotechnology and society, including regulation, patents, and controversies around genetically modified products.
Filled with engaging figures that strongly support the explanations in the text, Molecular Biotechnology: Principles and Applications of Recombinant DNA presents difficult scientific concepts and technically challenging methods in clear, crisp prose.

This excellent textbook is ideal for undergraduate and graduate courses in introductory biotechnology, as well as, courses dedicated to medical, agricultural, environmental, and industrial biotechnology applications.

دانلود کتاب مولکولی بیوتکنولوژی : اصول و کاربردها از DNA نوترکیب نسخه پنجم

از سال 1994 ، بیوتکنولوژی مولکولی: اصول و کاربردهای DNA نوترکیب دانشجویان را به دنیای سریع در حال تغییر بیوتکنولوژی مولکولی معرفی کرده است. با هر نسخه ، نویسندگان به طور گسترده ای کتاب را به روز کرده اند تا با بسیاری از تکنیک های جدید در جداسازی و تقویت ژن ، سنتز و توالی اسید نوکلئیک ، ویرایش ژن و کاربردهای آنها در بیوتکنولوژی همگام باشد. در این نسخه ، نویسندگان برنارد آر. گلیك و چریل ال پاتن این سنت را ادامه داده اند ، اما سازمان كتاب را نیز مورد بررسی قرار داده اند.

جزئیات روش های اساسی زیست شناسی مولکولی و تکنیک های مهندسی پروتئین نوترکیب را شرح دهید ، که پایه علمی محکمی را برای بقیه کتاب در اختیار دانشجویان قرار می دهد.
فرآیندهای بیوتکنولوژی بیولوژیکی مولکولی و موفقیتهای آن در پزشکی ، تجمع زیستی ، تولید مواد اولیه ، سوخت های زیستی و کشاورزی را ارائه دهید.
تقاطع بیوتکنولوژی مولکولی و جامعه ، از جمله مقررات ، اختراعات ثبت اختراعات و اختلاف نظرها درباره محصولات اصلاح شده ژنتیکی را بررسی کنید.

پر از چهره های جذاب که به شدت از توضیحات موجود در متن پشتیبانی می کنند ، بیوتکنولوژی مولکولی: اصول و کاربردهای DNA نوترکیب مفاهیم علمی دشوار و روشهای فنی چالش برانگیز را در نثر واضح و واضح ارائه می دهد.

این کتاب درسی عالی برای دوره های کارشناسی و کارشناسی ارشد در بیوتکنولوژی مقدماتی و همچنین دوره های اختصاصی به برنامه های کاربردی بیوتکنولوژی پزشکی ، کشاورزی ، زیست محیطی و صنعتی مناسب است.

فهرست مطالب PDF کتاب Molecular Biotechnology Principles and Applications نسخه 5

Table Of Contents
Cover
Contents
Preface to the Fifth Edition
1. The Development of Molecular Biotechnology
Emergence of Molecular Biotechnology
Recombinant DNA Technology
Commercialization of Molecular Biotechnology
Concerns and Consequences
Summary
References
Review Questions
2. Fundamental Technologies
Molecular Cloning
Preparation of DNA for Cloning
Insertion of Target DNA into a Plasmid Vector
Transformation and Selection of Cloned DNA in a Bacterial Host
Cloning Eukaryotic Genes
Recombinational Cloning
Genomic Libraries
Genome Engineering Using CRISPR Technology
Polymerase Chain Reaction
Amplification of DNA Using PCR
Cloning PCR Products
Quantitative PCR
Chemical Synthesis of DNA
Synthesis of Oligonucleotides
Assembling Oligonucleotides into Genes
Gene Synthesis by PCR
DNA Sequencing Technologies
Dideoxynucleotide Procedure
Pyrosequencing
Sequencing Using Reversible Chain Terminators
Sequencing by Single Molecule Synthesis
Sequencing Whole Genomes
Preparation of Genomic DNA Sequencing Libraries
High-Throughput Next-Generation Sequencing Strategies
Genome Sequence Assembly
Sequencing Metagenomes
Genomics
Transcriptomics
DNA Microarrays
RNA Sequencing
Proteomics
Identification of Proteins
Protein Expression Profiling
Protein−Protein Interactions
Metabolomics
Summary
References
Review Questions
3. Production of Recombinant Proteins
Protein Production in Prokaryotic Hosts
Regulation of Transcription
Increasing Translation Efficiency
Increasing Protein Stability
Facilitating Protein Folding
Decreasing Protein Degradation
Increasing Protein Secretion
Secretion into the Periplasm
Secretion into the Medium
Facilitating Protein Purification
DNA Integration into the Host Chromosome
Heterologous Protein Production in Eukaryotic Cells
Posttranslational Modification of Eukaryotic Proteins
General Features of Eukaryotic Expression Systems
Yeast Expression Systems
Saccharomyces cerevisiae Expression Vectors
Secretion of Heterologous Proteins by S. cerevisiae
Other Yeast Expression Systems
Baculovirus–Insect Cell Expression Systems
Baculovirus Expression Vectors
Mammalian Glycosylation and Processing of Proteins in Insect Cells
Mammalian Cell Expression Systems
Vector Design
Selectable Markers for Mammalian Expression Vectors
Engineering Mammalian Cell Hosts for Enhanced Productivity
Chromosomal Integration and Environment
Protein Engineering
Directed Mutagenesis
Site-Directed Mutagenesis by Overlap Extension PCR
Site-Directed Mutagenesis by Inverse PCR
Mutant Proteins with Unusual Amino Acids
Random Mutagenesis
Error-Prone PCR
Random Insertion/Deletion Mutagenesis
Random Mutagenesis with Degenerate Oligonucleotide Primers
DNA Shuffling
Examples of Protein Engineering
Increasing Protein Stability
Modifying Protein Specificity
Modifying Cofactor Requirements
Decreasing Protease Sensitivity
Summary
References
Review Questions
4. Molecular Diagnostics
Immunological Approaches to Detect Protein Biomarkers
Antibodies
Structure of Antibodies
Polyclonal Antibodies
Monoclonal Antibodies
Agglutination
Enzyme-Linked Immunosorbent Assays
Measuring Disease-Associated Proteins by Sandwich ELISA
Diagnosing Autoimmune Diseases using an Indirect ELISA
Protein Arrays to Detect Polygenic Diseases
Immunoassays for Protein Conformation-Specific Disorders
DNA-Based Diagnostic Approaches
Hybridization Probes
Allele-Specific Hybridization
Oligonucleotide Ligation Assay
Padlock Probes
Molecular Beacons
PCR-Based Detection Methods
Allele-Specific PCR
TaqMan PCR for SNP Genotypiing
Real-Time PCR to Detect Infectious Disease
Forensic Analysis Using PCR-Amplified Microsatellites
DNA Microarrays
Detection of Multiple Alleles
Detection of Epigenetic Markers
Ancestry Determination
Animal Species Determination
Whole Genome Sequencing to Assess Genetic Disease Risk
Detecting RNA Signatures of Disease
Detection of Disease-Associated Changes in Gene Expression Using Microarrays
Detection of RNA Signatures of Antibiotic Resistance in Bacteria
Detection of miRNA Signatures of Cancers
Biofluorescent and Bioluminescent Systems
Fluorescent Proteins
Luciferase
Microbial Biosensors
Summary
References
Review Questions
5. Protein Therapeutics
Pharmaceuticals
Human Interferons
Human Growth Hormone
Tumor Necrosis Factor Alpha
Extending Protein Half-Life
Enzymes
DNase I
Alginate Lyase
Phenylalanine Ammonia Lyase
α1-Antitrypsin
Glycosidases
Masking Nonhuman Epitopes
Engineered Bacteriophages
Targeting Mitochondria
Lactic Acid Bacteria
Interleukin-10
Leptin
An HIV Inhibitor
Insulin
Recombinant Antibodies
Hybrid Human–Mouse Monoclonal Antibodies
Human Monoclonal Antibodies
Antibody Fragments
Combinatorial Libraries of Antibody Fragments
A Combinatorial Library of Full-Length Antibodies
Shuffling CDR Sequences
Dual-Variable Domain Antibodies
Anticancer Antibodies
Antibodies Against Various Diseases
Antiobesity Antibodies
Enhanced Antibody Half-Life
Summary
References
Review Questions
6. Nucleic Acids as Therapeutic Agents
Targeting Specific mRNA and DNA Sequences
Antisense RNA
Aptamers
Ribozymes and DNAzymes
Interfering RNA
Zinc Finger Nucleases
CRISPR-Cas System
Nanozymes
Nanoparticles
Viral Delivery Systems
Nonviral Delivery Systems
Direct Injection
Lipids
Bacteria
Dendrimers
Antibodies
Aptamers
Transposons
Gene Therapy
Prodrug Activation Therapy
Promoterless Gene Targeting
Summary
References
Review Questions
7. Vaccines
Vaccination
Current and Future Vaccines
Subunit Vaccines
Herpes Simplex Virus
Cholera
SARS
Staphylococcus aureus
Human Papillomavirus
Foot-and-Mouth Disease
Streptococcus
Delivery
Peptide Vaccines
Malaria
Genetic Immunization: DNA Vaccines
Delivery
Cancer
Dental Caries
Attenuated Vaccines
Herpes Simplex Virus
Cholera
Salmonella Species
Leishmania Species
Vector Vaccines
Vaccines Directed against Viruses
Dengue Virus
Ebola Virus
Vaccines Directed against Bacteria
Tuberculosis
Bacteria as Antigen Delivery Systems
Cholera
Helicobacter pylori
Monoclonal Antibody Passive Immunity
Influenza Virus
Summary
References
Review Questions
8. Industrial and Environmental Uses of Recombinant Microorganisms
Restriction Endonucleases
Small Biological Molecules
L-Ascorbic Acid
Indigo
Amino Acids
L-Cysteine
L-Valine
L-Citrulline
Modified Corynebacterium
Lycopene
Antibiotics
Antibiotic Biosynthesis Genes
Engineering Polyketide Antibiotics
Unique Antibiotics
Overproducing Antibiotics
Biopolymers
Xanthan Gum
Polyhydroxyalkanoates
Microbial Degradation of Xenobiotics
Genetic Engineering of Biodegradative Pathways
Manipulation by Transfer of Plasmids
Manipulation by Gene Alteration
Utilization of Starch and Sugars
Commercial Production of Fructose and Alcohol
Increasing Alcohol Production
Alcohol Tolerance
Improving Fructose Production
Utilization of Cellulose and Hemicellulose
Lignocellulosics
Cellulase Genes
Direct Conversion of Biomass to Ethanol
Zymomonas mobilis
Lipids from Cyanobacteria
Hydrogen Production
Summary
References
Review Questions
9. Large-Scale Production of Proteins from Recombinant Microorganisms
Principles of Microbial Growth
Batch Fermentation
Fed-Batch Fermentation
Continuous Fermentation
Maximizing the Efficiency of the Fermentation Process
High-Density Cell Cultures
Increasing Plasmid Stability
Quiescent E. Coli Cells
Protein Secretion
Reducing Acetate
Bioreactors
Typical Large-Scale Fermentation Systems
Two-Stage Fermentation in Tandem Airlift Reactors
Two-Stage Fermentation in a Single Stirred-Tank Reactor
Batch versus Fed-Batch Fermentation
Harvesting Microbial Cells
Disrupting Microbial Cells
Downstream Processing
Protein Solubilization
Utilizing an Immobilized Enzyme
Magnetic Separation of Proteins
Large-Scale Production of Plasmid DNA
Summary
References
Review Questions
10. Genetic Engineering of Plants: Methodology
Plant Transformation with the Ti Plasmid of A. Tumefaciens
Ti Plasmid–Derived Vector Systems
Microprojectile Bombardment
Chloroplast Engineering
Very High Level Protein Expression
Use of Reporter Genes in Transformed Plant Cells
Manipulation of Gene Expression in Plants
Transient Gene Expression
Plant Promoters
Targeted Gene Editing
Facilitating Protein Purification
Oleosins
Rhizosecretion
Protein Glycosylation
Production of Marker-Free Transgenic Plants
Removing Marker Genes from Nuclear DNA
Removing Marker Genes from Chloroplast DNA
Summary
References
Review Questions
11. Transgenic Plants
Insect Resistance
B. thuringiensis Insecticidal Toxin
Increasing Expression of the B. thuringiensis Protoxin
Other Strategies for Protecting Plants against Insects
Protease Inhibitors
α-Amylase Inhibitor
Cholesterol Oxidase
Vegetative Insecticidal Toxins
Other Proteins
RNA Interference
Preventing the Development of B. thuringiensis–Resistant Insects
Targeting Aphids
Virus Resistance
Viral Coat Protein–Mediated Protection
Protection by Expression of Other Genes
Single-Chain Antibodies
Micro-RNAs
CRISPR-Cas
Herbicide Resistance
Fungus and Bacterium Resistance
Salt and Drought Stress
Fruit Ripening and Flower Wilting
Modification of Plant Nutritional Content
Amino Acids
Lipids
Vitamins
Vitamin A
Folate
Iron
Gluten
Modification of Food Plant Taste and Appearance
Preventing Discoloration
Starch
Plants as Bioreactors
Antibodies
Poly(3-hydroxybutyric Acid)
Edible Vaccines
Plant Yield
Altering Lignin Content
Increasing Oxygen Content
Summary
References
Review Questions
12. Transgenic Animals
Transgenic Mice: Methodology
DNA Microinjection Method
Retroviral Vector Method
Engineered Embryonic Stem Cell Method
Conditional Gene Inactivation with the Cre-loxP Recombination System
Genome Editing with the CRISPR-Cas System
Gene Knockdown by RNA Interference
Transgenic Mice: Applications
Transgenic Disease Models: Alzheimer Disease
Transgenic Mice as Test Systems
Control of Transgene Expression
Conditional Control of Cell Death
Transgenic Livestock
Cloning Livestock by Somatic Cell Nuclear Transfer
Production of Pharmaceuticals
Production of Donor Organs
Disease Resistant Livestock
Improving Milk Quality
Improving Animal Production Traits
Transgenic Poultry
Transgenic Fish
Summary
References
Review Questions
13. Molecular Biotechnology and Society
Development of Guidelines for Recombinant DNA Research
Deliberate Release of Genetically Modified Microorganisms
Environmental Concerns
Regulations
Regulation of Genetically Modified Foods
Food Ingredients Produced by Genetically Engineered Microorganisms
Genetically Modified Crops
Genetically Engineered Livestock
Societal Concerns About Genetically Modified Foods
Alteration of Nutritional Content of Food
Potential for Introducing Toxins or Allergens into Food
Potential for Transferring Transgenes from Food to Humans or Intestinal Microorganisms
Controversy About the Labeling of Genetically Modified Foods
Impact of Genetically Engineered Crops on Biodiversity
Who Benefits from the Production of Genetically Modified Foods?
Environmental Benefits of Genetically Modified Crops
How do Views about Genetically Modified Organisms Impact Trade?
Regulation and Safety of Medical Products of Biotechnology
New Biological Drugs
Genetic and Genomic Testing
Economic Issues
Patenting Biotechnology
Patenting
Patenting in Different Countries
Patenting Nucleic Acid Sequences
Patenting Living Organisms
Patenting and Fundamental Research
Summary
References
Review Questions
Amino Acids of Proteins and Their Designations
Index